Gfi1 and Gfi1b Repress Rag Transcription in Plasmacytoid Dendritic Cells In Vitro
|Journal / Publication||PLoS ONE|
|Online published||24 Sep 2013|
|Publication status||Published - 2013|
|Link to Scopus||https://www.scopus.com/record/display.uri?eid=2-s2.0-84884535466&origin=recordpage|
Growth factor independence genes (Gfi1 and Gfi1b) repress recombination activating genes (Rag) transcription in developing B lymphocytes. Because all blood lineages originate from hematopoietic stem cells (HSCs) and different lineage progenitors have been shown to share transcription factor networks prior to cell fate commitment, we hypothesized that GFI family proteins may also play a role in repressing Rag transcription or a global lymphoid transcriptional program in other blood lineages. We tested the level of Rag transcription in various blood cells when Gfi1 and Gfi1b were deleted, and observed an upregulation of Rag expression in plasmacytoid dendritic cells (pDCs). Using microarray analysis, we observed that Gfi1 and Gfi1b do not regulate a lymphoid or pDC-specific transcriptional program. This study establishes a role for Gfi1 and Gfi1b in Rag regulation in a non-B lineage cell type.
- Animals, Computational Biology, DNA-Binding Proteins/genetics, Dendritic Cells/metabolism, Flow Cytometry, Gene Expression Regulation/genetics, Homeodomain Proteins/metabolism, Humans, Mice, Microarray Analysis, Proto-Oncogene Proteins/genetics, Real-Time Polymerase Chain Reaction, Repressor Proteins/genetics, Reverse Transcriptase Polymerase Chain Reaction, Transcription Factors/genetics
Gfi1 and Gfi1b Repress Rag Transcription in Plasmacytoid Dendritic Cells In Vitro. / Chow, Kwan T.; Schulz, Danae; McWhirter, Sarah M.; Schlissel, Mark S.In: PLoS ONE, Vol. 8, No. 9, e75891, 2013.