Gfi1 and Gfi1b Repress Rag Transcription in Plasmacytoid Dendritic Cells In Vitro

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

Detail(s)

Original languageEnglish
Article numbere75891
Journal / PublicationPLoS ONE
Volume8
Issue number9
Online published24 Sep 2013
Publication statusPublished - 2013
Externally publishedYes

Abstract


Growth factor independence genes (Gfi1 and Gfi1b) repress recombination activating genes (Rag) transcription in developing B lymphocytes. Because all blood lineages originate from hematopoietic stem cells (HSCs) and different lineage progenitors have been shown to share transcription factor networks prior to cell fate commitment, we hypothesized that GFI family proteins may also play a role in repressing Rag transcription or a global lymphoid transcriptional program in other blood lineages. We tested the level of Rag transcription in various blood cells when Gfi1 and Gfi1b were deleted, and observed an upregulation of Rag expression in plasmacytoid dendritic cells (pDCs). Using microarray analysis, we observed that Gfi1 and Gfi1b do not regulate a lymphoid or pDC-specific transcriptional program. This study establishes a role for Gfi1 and Gfi1b in Rag regulation in a non-B lineage cell type.


Research Area(s)

  • Animals, Computational Biology, DNA-Binding Proteins/genetics, Dendritic Cells/metabolism, Flow Cytometry, Gene Expression Regulation/genetics, Homeodomain Proteins/metabolism, Humans, Mice, Microarray Analysis, Proto-Oncogene Proteins/genetics, Real-Time Polymerase Chain Reaction, Repressor Proteins/genetics, Reverse Transcriptase Polymerase Chain Reaction, Transcription Factors/genetics

Citation Format(s)

Gfi1 and Gfi1b Repress Rag Transcription in Plasmacytoid Dendritic Cells In Vitro. / Chow, Kwan T.; Schulz, Danae; McWhirter, Sarah M.; Schlissel, Mark S.

In: PLoS ONE, Vol. 8, No. 9, e75891, 2013.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal