TY - JOUR
T1 - Genotoxicity investigation of a cyanobacterial toxin, cylindrospermopsin
AU - Shen, Xiaoyun
AU - Lam, P. K S
AU - Shaw, G. R.
AU - Wickramasinghe, W.
PY - 2002/10/1
Y1 - 2002/10/1
N2 - Cylindrospermopsin (CYN), a potent cyanobacterial hepatotoxin produced by Cylindrospermopsis raciborskii and other cyanobacteria, is regularly found in water supplies in many parts of the world, and has been associated with the intoxication of humans and livestock. In this study, Balb/c mice were injected via the intraperitoneal (IP) route with a single dose of 0.2mg/kg CYN. Animals were sacrificed at 6, 12, 24, 48 and 72h. DNA was isolated from the mouse livers, and examined for strand breakage by alkaline gel electrophoresis (pH 12). Significant DNA strand breakage was observed in the mouse liver exposed to CYN, suggesting that induction of DNA strand breakage is probably one of the key mechanisms for CYN genotoxicity. © 2002 Elsevier Science Ltd. All rights reserved.
AB - Cylindrospermopsin (CYN), a potent cyanobacterial hepatotoxin produced by Cylindrospermopsis raciborskii and other cyanobacteria, is regularly found in water supplies in many parts of the world, and has been associated with the intoxication of humans and livestock. In this study, Balb/c mice were injected via the intraperitoneal (IP) route with a single dose of 0.2mg/kg CYN. Animals were sacrificed at 6, 12, 24, 48 and 72h. DNA was isolated from the mouse livers, and examined for strand breakage by alkaline gel electrophoresis (pH 12). Significant DNA strand breakage was observed in the mouse liver exposed to CYN, suggesting that induction of DNA strand breakage is probably one of the key mechanisms for CYN genotoxicity. © 2002 Elsevier Science Ltd. All rights reserved.
KW - Alkaline gel electrophoresis
KW - Cylindrospermopsin
KW - DNA strand breaks
KW - Genotoxicity
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U2 - 10.1016/S0041-0101(02)00151-4
DO - 10.1016/S0041-0101(02)00151-4
M3 - RGC 21 - Publication in refereed journal
C2 - 12368121
SN - 0041-0101
VL - 40
SP - 1499
EP - 1501
JO - Toxicon
JF - Toxicon
IS - 10
ER -
