Genomic insights into the emergence and spread of NDM-1-producing Vibrio spp. isolates in China

Zhiwei Zheng; Yating Xu; Lianwei Ye; Edward Wai Chi Chan; Sheng Chen

doi:10.1093/jac/dkac276

Genomic insights into the emergence and spread of NDM-1-producing Vibrio spp. isolates in China

Zhiwei Zheng
, Yating Xu
, Lianwei Ye
, Edward Wai Chi Chan
, Sheng Chen^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

12 Citations (Scopus)

Abstract

Background: Carbapenemase-producing Vibrio spp., which exhibit an XDR phenotype, have become increasingly prevalent and pose a severe threat to public health.

Objectives: To investigate the genetic characteristics of NDM-1-producing Vibrio spp. isolates and the dissemination mechanisms of bla_NDM-1 in Vibrio.

Methods: A total of 1363 non-duplicate Vibrio spp. isolates collected from shrimp samples in China were subjected to antimicrobial susceptibility tests and screened for bla_NDM-1. The bla_NDM-1-positive isolates were further characterized by PFGE, MLST, conjugation and WGS using Illumina and Nanopore platforms. Plasmid stability and fitness cost were assessed using Escherichia coli J53, Klebsiella pneumoniae Kpt80 and Salmonella spp. SA2051 as recipient strains.

Results: In total, 13 bla_NDM-1-positive isolates were identified, all exhibiting MDR. WGS analysis revealed that the 13 bla_NDM-1 genes were all associated with a derivative of Tn125. Plasmid analysis revealed that six bla_NDM-1 genes were located in IncC plasmids and the other seven were carried by plasmids of two different novel types. Conjugation and plasmid stability assays showed that only the IncC plasmids could be transferred to all the recipient strains and could be stably maintained in the hosts.

Conclusions: The emergence of the novel plasmids has contributed to the variable genetic contexts of bla_NDM-1 in Vibrio spp. and IncC plasmids harbouring the bla_NDM-1 gene could facilitate the spread of such genes between Vibrio spp. and other zoonotic pathogens, leading to a rapid dissemination of bla_NDM-1 in bacterial pathogens worldwide.

Original language	English
Pages (from-to)	3039-3049
Number of pages	11
Journal	The Journal of antimicrobial chemotherapy
Volume	77
Issue number	11
Online published	18 Aug 2022
DOIs	https://doi.org/10.1093/jac/dkac276
Publication status	Published - Nov 2022

Bibliographical note

Funding

The research was supported by the Guangdong Major Project of Basic and Applied Basic Research (2020B0301030005) and an NSFC/RGC grant (NSFC-RGC, N_PolyU521/18) from the National Natural Science Fund in China and Research Grant Council of the Government of Hong Kong SAR.

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@article{a586293ff4884273880c8e63a8ac4ce1,

title = "Genomic insights into the emergence and spread of NDM-1-producing Vibrio spp. isolates in China",

abstract = "Background: Carbapenemase-producing Vibrio spp., which exhibit an XDR phenotype, have become increasingly prevalent and pose a severe threat to public health.Objectives: To investigate the genetic characteristics of NDM-1-producing Vibrio spp. isolates and the dissemination mechanisms of blaNDM-1 in Vibrio.Methods: A total of 1363 non-duplicate Vibrio spp. isolates collected from shrimp samples in China were subjected to antimicrobial susceptibility tests and screened for blaNDM-1. The blaNDM-1-positive isolates were further characterized by PFGE, MLST, conjugation and WGS using Illumina and Nanopore platforms. Plasmid stability and fitness cost were assessed using Escherichia coli J53, Klebsiella pneumoniae Kpt80 and Salmonella spp. SA2051 as recipient strains.Results: In total, 13 blaNDM-1-positive isolates were identified, all exhibiting MDR. WGS analysis revealed that the 13 blaNDM-1 genes were all associated with a derivative of Tn125. Plasmid analysis revealed that six blaNDM-1 genes were located in IncC plasmids and the other seven were carried by plasmids of two different novel types. Conjugation and plasmid stability assays showed that only the IncC plasmids could be transferred to all the recipient strains and could be stably maintained in the hosts.Conclusions: The emergence of the novel plasmids has contributed to the variable genetic contexts of blaNDM-1 in Vibrio spp. and IncC plasmids harbouring the blaNDM-1 gene could facilitate the spread of such genes between Vibrio spp. and other zoonotic pathogens, leading to a rapid dissemination of blaNDM-1 in bacterial pathogens worldwide.",

author = "Zhiwei Zheng and Yating Xu and Lianwei Ye and Chan, \{Edward Wai Chi\} and Sheng Chen",

note = "{\textcopyright} The Author(s) 2022. Published by Oxford University Press on behalf of British Society for Antimicrobial Chemotherapy. All rights reserved. For permissions, please e-mail: [email protected].",

year = "2022",

month = nov,

doi = "10.1093/jac/dkac276",

language = "English",

volume = "77",

pages = "3039--3049",

journal = "The Journal of antimicrobial chemotherapy",

issn = "0305-7453",

publisher = "Oxford University Press",

number = "11",

}

TY - JOUR

T1 - Genomic insights into the emergence and spread of NDM-1-producing Vibrio spp. isolates in China

AU - Zheng, Zhiwei

AU - Xu, Yating

AU - Ye, Lianwei

AU - Chan, Edward Wai Chi

AU - Chen, Sheng

PY - 2022/11

Y1 - 2022/11

N2 - Background: Carbapenemase-producing Vibrio spp., which exhibit an XDR phenotype, have become increasingly prevalent and pose a severe threat to public health.Objectives: To investigate the genetic characteristics of NDM-1-producing Vibrio spp. isolates and the dissemination mechanisms of blaNDM-1 in Vibrio.Methods: A total of 1363 non-duplicate Vibrio spp. isolates collected from shrimp samples in China were subjected to antimicrobial susceptibility tests and screened for blaNDM-1. The blaNDM-1-positive isolates were further characterized by PFGE, MLST, conjugation and WGS using Illumina and Nanopore platforms. Plasmid stability and fitness cost were assessed using Escherichia coli J53, Klebsiella pneumoniae Kpt80 and Salmonella spp. SA2051 as recipient strains.Results: In total, 13 blaNDM-1-positive isolates were identified, all exhibiting MDR. WGS analysis revealed that the 13 blaNDM-1 genes were all associated with a derivative of Tn125. Plasmid analysis revealed that six blaNDM-1 genes were located in IncC plasmids and the other seven were carried by plasmids of two different novel types. Conjugation and plasmid stability assays showed that only the IncC plasmids could be transferred to all the recipient strains and could be stably maintained in the hosts.Conclusions: The emergence of the novel plasmids has contributed to the variable genetic contexts of blaNDM-1 in Vibrio spp. and IncC plasmids harbouring the blaNDM-1 gene could facilitate the spread of such genes between Vibrio spp. and other zoonotic pathogens, leading to a rapid dissemination of blaNDM-1 in bacterial pathogens worldwide.

AB - Background: Carbapenemase-producing Vibrio spp., which exhibit an XDR phenotype, have become increasingly prevalent and pose a severe threat to public health.Objectives: To investigate the genetic characteristics of NDM-1-producing Vibrio spp. isolates and the dissemination mechanisms of blaNDM-1 in Vibrio.Methods: A total of 1363 non-duplicate Vibrio spp. isolates collected from shrimp samples in China were subjected to antimicrobial susceptibility tests and screened for blaNDM-1. The blaNDM-1-positive isolates were further characterized by PFGE, MLST, conjugation and WGS using Illumina and Nanopore platforms. Plasmid stability and fitness cost were assessed using Escherichia coli J53, Klebsiella pneumoniae Kpt80 and Salmonella spp. SA2051 as recipient strains.Results: In total, 13 blaNDM-1-positive isolates were identified, all exhibiting MDR. WGS analysis revealed that the 13 blaNDM-1 genes were all associated with a derivative of Tn125. Plasmid analysis revealed that six blaNDM-1 genes were located in IncC plasmids and the other seven were carried by plasmids of two different novel types. Conjugation and plasmid stability assays showed that only the IncC plasmids could be transferred to all the recipient strains and could be stably maintained in the hosts.Conclusions: The emergence of the novel plasmids has contributed to the variable genetic contexts of blaNDM-1 in Vibrio spp. and IncC plasmids harbouring the blaNDM-1 gene could facilitate the spread of such genes between Vibrio spp. and other zoonotic pathogens, leading to a rapid dissemination of blaNDM-1 in bacterial pathogens worldwide.

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UR - https://app-overton-io.ezproxy.cityu.edu.hk/articles.php?query=10.1093/jac/dkac276

U2 - 10.1093/jac/dkac276

DO - 10.1093/jac/dkac276

M3 - RGC 21 - Publication in refereed journal

C2 - 35978475

SN - 0305-7453

VL - 77

SP - 3039

EP - 3049

JO - The Journal of antimicrobial chemotherapy

JF - The Journal of antimicrobial chemotherapy

IS - 11

ER -

Genomic insights into the emergence and spread of NDM-1-producing Vibrio spp. isolates in China

Abstract

Bibliographical note

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