Genome Architecture Mediates Transcriptional Control of Human Myogenic Reprogramming

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

17 Scopus Citations
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Author(s)

  • Sijia Liu
  • Haiming Chen
  • Scott Ronquist
  • Laura Seaman
  • Nicholas Ceglia
  • Walter Meixner
  • Pin-Yu Chen
  • Gerald Higgins
  • Pierre Baldi
  • Alfred Hero
  • Lindsey A. Muir
  • Indika Rajapakse

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)232-246
Journal / PublicationiScience
Volume6
Online published8 Aug 2018
Publication statusPublished - 31 Aug 2018

Link(s)

Abstract

Genome architecture has emerged as a critical element of transcriptional regulation, although its role in the control of cell identity is not well understood. Here we use transcription factor (TF)-mediated reprogramming to examine the interplay between genome architecture and transcriptional programs that transition cells into the myogenic identity. We recently developed new methods for evaluating the topological features of genome architecture based on network centrality. Through integrated analysis of these features of genome architecture and transcriptome dynamics during myogenic reprogramming of human fibroblasts we find that significant architectural reorganization precedes activation of a myogenic transcriptional program. This interplay sets the stage for a critical transition observed at several genomic scales reflecting definitive adoption of the myogenic phenotype. Subsequently, TFs within the myogenic transcriptional program participate in entrainment of biological rhythms. These findings reveal a role for topological features of genome architecture in the initiation of transcriptional programs during TF-mediated human cellular reprogramming.

Research Area(s)

  • SKELETAL-MUSCLE, STEM-CELLS, TOPOLOGICAL DOMAINS, MYOD, EXPRESSION, DIFFERENTIATION, FAMILY, ORGANIZATION, PRINCIPLES, ALGORITHM

Citation Format(s)

Genome Architecture Mediates Transcriptional Control of Human Myogenic Reprogramming. / Liu, Sijia; Chen, Haiming; Ronquist, Scott et al.
In: iScience, Vol. 6, 31.08.2018, p. 232-246.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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