Genetic Characterization of blaCTX-M-55-Bearing Plasmids Harbored by Food-Borne Cephalosporin-Resistant Vibrio parahaemolyticus Strains in China

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Zhiwei Zheng
  • Ruichao Li
  • Lianwei Ye
  • Edward Wai-Chi Chan
  • Xiaodong Xia

Detail(s)

Original languageEnglish
Article number1338
Journal / PublicationFrontiers in Microbiology
Volume10
Online published18 Jun 2019
Publication statusPublished - Jun 2019
Externally publishedYes

Abstract

This study aims to investigate and compare the complete nucleotide sequences of the multidrug resistance plasmids pVb0267 and pVb0499, which were recovered from foodborne Vibrio parahaemolyticus isolates, and analyze the genetic environment of blaCTX-M-55 to provide insight into the dissemination mechanisms of this resistance element. Analysis of the sequences of plasmids pVb0267 (166, 467 bp) and pVb0499 (192, 739 bp) revealed that the backbones of these two plasmids exhibited a high degree of similarity with pR148, a recognized type 1a IncC plasmid recovered from Aeromonas hydrophila (99% identity). The resistance genes, found in both plasmids, included qacH, aadB, arr2, blaOXA-10, aadA1, sul1, tet(A), and blaCTX M-55, which were mostly arranged in a specific region designated ARI-A. Plasmid pVb0499 was found to possess a larger size of ARI-A than pVb0267, which lacked a mer determination region, a qnrA segment, an aacC3 gene and several mobility-encoding genes. Comparative analysis of resistance island (RI) of these plasmids and others revealed the potential evolution route of these RI sequences. In conclusion, plasmids harboring the blaCTX M-55 gene has been recovered in Vibrio parahaemolyticus strains of food origin. It is alarming to find that IncC plasmids harboring resistance islands are disseminating in aquatic bacterial strains. The continuous evolution of resistance genes in conjugative plasmid in aquatic bacteria could be due to bacterial adaption to aquaculture environment, where antibiotics were increasingly used.

Research Area(s)

  • blaCTX-M-55, Cephalosporin resistance, Conjugative plasmid, IncC plasmid, V. parahaemolyticus

Citation Format(s)

Genetic Characterization of blaCTX-M-55-Bearing Plasmids Harbored by Food-Borne Cephalosporin-Resistant Vibrio parahaemolyticus Strains in China. / Zheng, Zhiwei; Li, Ruichao; Ye, Lianwei; Chan, Edward Wai-Chi; Xia, Xiaodong; Chen, Sheng.

In: Frontiers in Microbiology, Vol. 10, 1338, 06.2019.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review