TY - JOUR
T1 - Genetic characterization and evolutionary analysis of 4 Newcastle disease virus isolate full genomes from waterbirds in South China during 2003-2007
AU - Cai, Shaoxin
AU - Li, Jun
AU - Wong, Mabel Ting
AU - Jiao, Peirong
AU - Fan, Huiying
AU - Liu, Dawei
AU - Liao, Ming
AU - Jiang, Jingwei
AU - Shi, Mang
AU - Lam, Tommy Tsan-Yuk
AU - Ren, Tao
AU - Leung, Frederick Chi-Ching
PY - 2011/8/26
Y1 - 2011/8/26
N2 - Complete genomes of four Newcastle disease virus (NDV) strains, isolated from ducks and wild birds in Guangdong province of China from 2003 to 2007, were sequenced and analyzed in this study. Pathogenicity tests in chicken embryos and chickens illustrate that D3 and R8 are lentogenic, and W4 and P4 are mesogenic strains. Phylogenetic analysis using all six genes provides a high resolution profile for genotype designation as genotype I for D3 and R8 strains and genotype VI for W4 and P4 strains. In addition, molecular dating based on different genes suggests that D3 and R8 diverged from their common ancestor at around 1998; W4 and P4 diverged from their common ancestor at around 1999. Subsequent selective pressure analysis displayed specific traits of genes evolution in all 4 strains since their divergence from the recent common ancestor. Furthermore, the geographic origins of 4 strains were deduced to be from Europe via two independent introduction events by phylogeographical analysis. This provides insights to the potential influence of waterfowl migration on NDV epidemiology.
AB - Complete genomes of four Newcastle disease virus (NDV) strains, isolated from ducks and wild birds in Guangdong province of China from 2003 to 2007, were sequenced and analyzed in this study. Pathogenicity tests in chicken embryos and chickens illustrate that D3 and R8 are lentogenic, and W4 and P4 are mesogenic strains. Phylogenetic analysis using all six genes provides a high resolution profile for genotype designation as genotype I for D3 and R8 strains and genotype VI for W4 and P4 strains. In addition, molecular dating based on different genes suggests that D3 and R8 diverged from their common ancestor at around 1998; W4 and P4 diverged from their common ancestor at around 1999. Subsequent selective pressure analysis displayed specific traits of genes evolution in all 4 strains since their divergence from the recent common ancestor. Furthermore, the geographic origins of 4 strains were deduced to be from Europe via two independent introduction events by phylogeographical analysis. This provides insights to the potential influence of waterfowl migration on NDV epidemiology.
KW - Evolutionary analysis
KW - Newcastle disease virus
KW - Phylogenetic tree
KW - Phylogenomic tree
KW - Phylogeographic
KW - Selective pressure
KW - Waterfowls
UR - http://www.scopus.com/inward/record.url?scp=79960403262&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-79960403262&origin=recordpage
U2 - 10.1016/j.vetmic.2011.04.014
DO - 10.1016/j.vetmic.2011.04.014
M3 - RGC 21 - Publication in refereed journal
C2 - 21565454
SN - 0378-1135
VL - 152
SP - 46
EP - 54
JO - Veterinary Microbiology
JF - Veterinary Microbiology
IS - 1-2
ER -