Genetic and Functional Characterization of a Conjugative KpVP-2-Type Virulence Plasmid From a Clinical Klebsiella pneumoniae Strain

Xuemei Yang; Xiaoxuan Liu; Yating Xu; Chen Yang; Edward Wai-Chi Chan; Hoi-ping Shum; Sheng Chen

doi:10.3389/fmicb.2022.914884

Genetic and Functional Characterization of a Conjugative KpVP-2-Type Virulence Plasmid From a Clinical Klebsiella pneumoniae Strain

Xuemei Yang, Xiaoxuan Liu, Yating Xu, Chen Yang, Edward Wai-Chi Chan, Hoi-ping Shum, Sheng Chen^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

8 Citations (Scopus)

66 Downloads (CityUHK Scholars)

Abstract

The main mechanism of virulence in Klebsiella pneumoniae is the acquisition of K. pneumoniae virulence plasmids (KpVPs), which include two dominant types, namely, KpVP-1 (carrying iuc1, iro1, rmpA, and rmpA2) and KpVP-2 (carrying iuc2, iro2, and rmpA). Both are non-conjugative and associated with different hypervirulent clones. In contrast to KpVP-1 reported in K1, K2, and other serotypes of K. pneumoniae, KpVP-2 was only reported in K2 strains and rarely characterized. In this study, we identified a conjugative KpVP-2-type virulence plasmid from a clinical hypervirulent K. pneumoniae strain. This plasmid was generated by the integration of conjugative transfer genes into the KpVP-2-type plasmid Kp52.145 II and could be readily conjugated to Escherichia coli strain EC600 and K. pneumoniae strains of various types which are clinically existing, mediating hypervirulence. Furthermore, this kind of conjugative KpVP-2-type virulence plasmid has been disseminated in clinical settings in Hong Kong and other regions of the world. The generation of conjugative virulence plasmid may promote its transmission and explain the evolution of this type of virulence plasmid.

Original language	English
Article number	914884
Journal	Frontiers in Microbiology
Volume	13
Online published	22 Jul 2022
DOIs	https://doi.org/10.3389/fmicb.2022.914884
Publication status	Published - Jul 2022

Funding

The research was supported by Guangdong Major Project of Basic and Applied Basic Research (2020B0301030005) and the NSFC/RGC grant (NSFC-RGC, N_PolyU521/18) from the National Natural Science Fund in China and the Research Grant Council of the Government of Hong Kong SAR.

Research Keywords

conjugative plasmid
Klebsiella pneumoniae
Klebsiella pneumoniae virulence plasmids type 2 (KpVP-2)
mobile genetic element
virulence

Publisher's Copyright Statement

This full text is made available under CC-BY 4.0. https://creativecommons.org/licenses/by/4.0/

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access Output

10.3389/fmicb.2022.914884

112835207.pdfFinal Published version, 1.83 MBLicence: CC BY

Other Access Options

Check CityUHK Library

Permanent Link

Right click to copy link

Cite this

@article{0812277e6cae4df6a582bf5e7642ef01,

title = "Genetic and Functional Characterization of a Conjugative KpVP-2-Type Virulence Plasmid From a Clinical Klebsiella pneumoniae Strain",

abstract = "The main mechanism of virulence in Klebsiella pneumoniae is the acquisition of K. pneumoniae virulence plasmids (KpVPs), which include two dominant types, namely, KpVP-1 (carrying iuc1, iro1, rmpA, and rmpA2) and KpVP-2 (carrying iuc2, iro2, and rmpA). Both are non-conjugative and associated with different hypervirulent clones. In contrast to KpVP-1 reported in K1, K2, and other serotypes of K. pneumoniae, KpVP-2 was only reported in K2 strains and rarely characterized. In this study, we identified a conjugative KpVP-2-type virulence plasmid from a clinical hypervirulent K. pneumoniae strain. This plasmid was generated by the integration of conjugative transfer genes into the KpVP-2-type plasmid Kp52.145 II and could be readily conjugated to Escherichia coli strain EC600 and K. pneumoniae strains of various types which are clinically existing, mediating hypervirulence. Furthermore, this kind of conjugative KpVP-2-type virulence plasmid has been disseminated in clinical settings in Hong Kong and other regions of the world. The generation of conjugative virulence plasmid may promote its transmission and explain the evolution of this type of virulence plasmid.",

keywords = "conjugative plasmid, Klebsiella pneumoniae, Klebsiella pneumoniae virulence plasmids type 2 (KpVP-2), mobile genetic element, virulence",

author = "Xuemei Yang and Xiaoxuan Liu and Yating Xu and Chen Yang and Chan, {Edward Wai-Chi} and Hoi-ping Shum and Sheng Chen",

year = "2022",

month = jul,

doi = "10.3389/fmicb.2022.914884",

language = "English",

volume = "13",

journal = "Frontiers in Microbiology",

issn = "1664-302X",

publisher = "Frontiers Media S.A.",

}

TY - JOUR

T1 - Genetic and Functional Characterization of a Conjugative KpVP-2-Type Virulence Plasmid From a Clinical Klebsiella pneumoniae Strain

AU - Yang, Xuemei

AU - Liu, Xiaoxuan

AU - Xu, Yating

AU - Yang, Chen

AU - Chan, Edward Wai-Chi

AU - Shum, Hoi-ping

AU - Chen, Sheng

PY - 2022/7

Y1 - 2022/7

N2 - The main mechanism of virulence in Klebsiella pneumoniae is the acquisition of K. pneumoniae virulence plasmids (KpVPs), which include two dominant types, namely, KpVP-1 (carrying iuc1, iro1, rmpA, and rmpA2) and KpVP-2 (carrying iuc2, iro2, and rmpA). Both are non-conjugative and associated with different hypervirulent clones. In contrast to KpVP-1 reported in K1, K2, and other serotypes of K. pneumoniae, KpVP-2 was only reported in K2 strains and rarely characterized. In this study, we identified a conjugative KpVP-2-type virulence plasmid from a clinical hypervirulent K. pneumoniae strain. This plasmid was generated by the integration of conjugative transfer genes into the KpVP-2-type plasmid Kp52.145 II and could be readily conjugated to Escherichia coli strain EC600 and K. pneumoniae strains of various types which are clinically existing, mediating hypervirulence. Furthermore, this kind of conjugative KpVP-2-type virulence plasmid has been disseminated in clinical settings in Hong Kong and other regions of the world. The generation of conjugative virulence plasmid may promote its transmission and explain the evolution of this type of virulence plasmid.

AB - The main mechanism of virulence in Klebsiella pneumoniae is the acquisition of K. pneumoniae virulence plasmids (KpVPs), which include two dominant types, namely, KpVP-1 (carrying iuc1, iro1, rmpA, and rmpA2) and KpVP-2 (carrying iuc2, iro2, and rmpA). Both are non-conjugative and associated with different hypervirulent clones. In contrast to KpVP-1 reported in K1, K2, and other serotypes of K. pneumoniae, KpVP-2 was only reported in K2 strains and rarely characterized. In this study, we identified a conjugative KpVP-2-type virulence plasmid from a clinical hypervirulent K. pneumoniae strain. This plasmid was generated by the integration of conjugative transfer genes into the KpVP-2-type plasmid Kp52.145 II and could be readily conjugated to Escherichia coli strain EC600 and K. pneumoniae strains of various types which are clinically existing, mediating hypervirulence. Furthermore, this kind of conjugative KpVP-2-type virulence plasmid has been disseminated in clinical settings in Hong Kong and other regions of the world. The generation of conjugative virulence plasmid may promote its transmission and explain the evolution of this type of virulence plasmid.

KW - conjugative plasmid

KW - Klebsiella pneumoniae

KW - Klebsiella pneumoniae virulence plasmids type 2 (KpVP-2)

KW - mobile genetic element

KW - virulence

UR - http://www.scopus.com/inward/record.url?scp=85135494416&partnerID=8YFLogxK

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85135494416&origin=recordpage

U2 - 10.3389/fmicb.2022.914884

DO - 10.3389/fmicb.2022.914884

M3 - RGC 21 - Publication in refereed journal

C2 - 35935210

SN - 1664-302X

VL - 13

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

M1 - 914884

ER -

Genetic and Functional Characterization of a Conjugative KpVP-2-Type Virulence Plasmid From a Clinical Klebsiella pneumoniae Strain

Abstract

Funding

Research Keywords

Publisher's Copyright Statement

UN SDGs

Access Output

Other Access Options

Permanent Link

Other Files and Links

Fingerprint

Cite this