Gene transfer and living release of transforming growth factor-β3 for cartilage tissue engineering applications

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Jinghua Hao
  • Yongchang Yao
  • Rohan R. Varshney
  • Laicheng Wang
  • Celine Prakash
  • Hao Li

Detail(s)

Original languageEnglish
Pages (from-to)273-280
Journal / PublicationTissue Engineering - Part C: Methods
Volume14
Issue number4
Publication statusPublished - 1 Dec 2008
Externally publishedYes

Abstract

Cartilage restoration continues to present a tremendous clinical challenge due to its nonvascular nature. Many studies have demonstrated that chondrogenesis of progenitor cells can be achieved in vitro by manual dose of growth factors; however, it remains a vital difficulty in feeding growth factors to implanted therapeutic cells in vivo. Herein, we constructed recombinant adenovirus encoding human transforming growth factor-β3 (hTGF-β3) and practiced it in rat bone marrow-derived mesenchymal stromal cells and articular chondrocytes for cartilage regeneration. Optimal viable transduction and transgenic hTGF-β3 production were achieved; consequently, positive expression of cartilage marker-collagen type II was enabled in the infected progenitors. We thus conclude that recombinant adenovirus encoding TGF-β3 gene has been successfully established and validated for cartilage tissue engineering applications. © Copyright 2008, Mary Ann Liebert, Inc.

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Citation Format(s)

Gene transfer and living release of transforming growth factor-β3 for cartilage tissue engineering applications. / Hao, Jinghua; Yao, Yongchang; Varshney, Rohan R.; Wang, Laicheng; Prakash, Celine; Li, Hao; Wang, Dong-An.

In: Tissue Engineering - Part C: Methods, Vol. 14, No. 4, 01.12.2008, p. 273-280.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review