Gene expression profiles showing hepatotoxicity in Balb/c mice exposed to cyclophosphamide and ifosfamide

AIM: To study the molecular mechanism of hepatotoxicity of cyclophosphamide and ifosfamide. METHODS: Utilizing toxicological microarray and animal model of cyclophosphamide (CP) and ifosfamide (Ifo) injured Balb/c mouse liver, which established by ourself, to observe the gene expression profiles at different time after drug treatment, and analyze the function of these differential expressed genes by hierarchical clustering and querying bioinformation databases. RESULTS: Multiple differential expressed genes were found. Some similar expression changes existed in gene expression profiles after treatments of two drugs, such as up-regulated protein synthesis associated genes, unbalanced anti-oxidation associated gene expression, abnormal immunity, cell cycle and apoptosis associated genes suggesting multiple common mechanisms involve hepatotoxicities of CP and Ifo. CONCLUSION: There are common molecular mechanisms in hepatotoxicity of CP and Ifo.