Gene coexpression network during ontogeny in the yellow fever mosquito, Aedes aegypti

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Yuqi Huang
  • Sihan Liu
  • Qiwen Huang
  • Biliang Zhang
  • Tianpeng Wang
  • Ziding Zhang
  • Chenghong Liao
  • Qian Han

Detail(s)

Original languageEnglish
Article number301
Journal / PublicationBMC Genomics
Volume24
Online published3 Jun 2023
Publication statusPublished - 2023

Link(s)

Abstract

Background The behaviors and ontogeny of Aedes aegypti are closely related to the spread of diseases caused by dengue (DENV), chikungunya (CHIKV), Zika (ZIKV), and yellow fever (YFV) viruses. During the life cycle, Ae. aegypti undergoes drastic morphological, metabolic, and functional changes triggered by gene regulation and other molecular mechanisms. Some essential regulatory factors that regulate insect ontogeny have been revealed in other species, but their roles are still poorly investigated in the mosquito.

Results Our study identified 6 gene modules and their intramodular hub genes that were highly associated with the ontogeny of Ae. aegypti in the constructed network. Those modules were found to be enriched in functional roles related to cuticle development, ATP generation, digestion, immunity, pupation control, lectins, and spermatogenesis. Additionally, digestion-related pathways were activated in the larvae and adult females but suppressed in the pupae. The integrated protein‒protein network also identified cilium-related genes. In addition, we verified that the 6 intramodular hub genes encoding proteins such as EcKinase regulating larval molt were only expressed in the larval stage. Quantitative RT‒PCR of the intramodular hub genes gave similar results as the RNA-Seq expression profile, and most hub genes were ontogeny-specifically expressed.

Conclusions The constructed gene coexpression network provides a useful resource for network-based data mining to identify candidate genes for functional studies. Ultimately, these findings will be key in identifying potential molecular targets for disease control.

© The Author(s) 2023.

Research Area(s)

  • Mosquito, Ontogeny, Aedes aegypti, Gene coexpression network, WGCNA

Bibliographic Note

© 2023. The Author(s).

Citation Format(s)

Gene coexpression network during ontogeny in the yellow fever mosquito, Aedes aegypti. / Lin, Zhinan; Huang, Yuqi; Liu, Sihan et al.
In: BMC Genomics, Vol. 24, 301, 2023.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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