Gas chromatography-mass spectrometry determination of matrine in human plasma

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

Detail(s)

Original languageEnglish
Pages (from-to)209-214
Journal / PublicationJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume808
Issue number2
Publication statusPublished - 5 Sep 2004
Externally publishedYes

Abstract

A method was developed for the quantification of matrine in human plasma using a liquid-liquid extraction procedure followed by gas-chromatography-mass spectrometry (GC/MS) analysis. Deuterated matrine, an internal standard of the analysis, was spiked into the plasma samples before extraction. Linear detection responses were obtained for matrine concentrations ranging from 10 to 500 ng/ml. The intra-day and inter-day precision ranged from 0.4 to 4.0% and 1.0-3.5%, respectively. The intra-day accuracy was between -7.3 and 4.5%. The limit of quantification for matrine was 23 ng/ml. The extraction efficiency averaged about 38%. The validated GC/MS method will be used to quantify matrine in human plasma samples collected in a clinical trial study. © 2004 Elsevier B.V. All rights reserved.

Research Area(s)

  • Matrine

Bibliographic Note

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Citation Format(s)

Gas chromatography-mass spectrometry determination of matrine in human plasma. / Sit, Dan S.T.; Gao, Guanghua; Law, Francis C.P.; Li, Paul C.H.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 808, No. 2, 05.09.2004, p. 209-214.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review