TY - JOUR
T1 - Fundamentals and applications of surface-enhanced Raman spectroscopy–based biosensors
AU - Li, Penghui
AU - Long, Feng
AU - Chen, Wanling
AU - Chen, Jing
AU - Chu, Paul K.
AU - Wang, Huaiyu
PY - 2020/3
Y1 - 2020/3
N2 - Surface-enhanced Raman scattering (SERS) has become a mature spectroscopic technique with the fast development in the past four decades, and its detection applications in the fields of chemistry, materials science, biochemistry and life sciences are rapidly expanding. In particular, advances in the design and construction of SERS-based biosensors have delivered tremendous development in the biological and biomedical sensing applications. Electromagnetic enhancement contributes dominantly to SERS enhancement, and the hotspot structures are crucial to sensitive and reproducible detection performance. SERS-based biosensors can be produced by direct and indirect techniques according to the sensing needs. In this mini-review, the enhancing mechanism and SERS structures are briefly described and the various common fabrication techniques are discussed. Recent state-of-the-art applications of SERS-based biosensors such as detection of ex vivo biofluids and biomolecules such as proteins, DNAs and microRNAs, as well as monitoring of cellular properties including pH, temperature and ion concentrations, are reviewed.
AB - Surface-enhanced Raman scattering (SERS) has become a mature spectroscopic technique with the fast development in the past four decades, and its detection applications in the fields of chemistry, materials science, biochemistry and life sciences are rapidly expanding. In particular, advances in the design and construction of SERS-based biosensors have delivered tremendous development in the biological and biomedical sensing applications. Electromagnetic enhancement contributes dominantly to SERS enhancement, and the hotspot structures are crucial to sensitive and reproducible detection performance. SERS-based biosensors can be produced by direct and indirect techniques according to the sensing needs. In this mini-review, the enhancing mechanism and SERS structures are briefly described and the various common fabrication techniques are discussed. Recent state-of-the-art applications of SERS-based biosensors such as detection of ex vivo biofluids and biomolecules such as proteins, DNAs and microRNAs, as well as monitoring of cellular properties including pH, temperature and ion concentrations, are reviewed.
KW - Surface-enhanced Raman scattering
KW - Biomolecules
KW - Cellular
KW - Biosensor
KW - Detection
UR - http://www.scopus.com/inward/record.url?scp=85073764499&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85073764499&origin=recordpage
U2 - 10.1016/j.cobme.2019.08.008
DO - 10.1016/j.cobme.2019.08.008
M3 - RGC 21 - Publication in refereed journal
SN - 2468-4511
VL - 13
SP - 51
EP - 59
JO - Current Opinion in Biomedical Engineering
JF - Current Opinion in Biomedical Engineering
ER -