Functional role of TRPV4-KCa2.3 signaling in vascular endothelial cells in Normal and streptozotocin-induced diabetic rats

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Xin Ma
  • Juan Du
  • Peng Zhang
  • Jianxin Deng
  • Jie Liu
  • Francis Fu-Yuen Lam
  • Ronald A. Li
  • Jian Jin
  • Xiaoqiang Yao

Detail(s)

Original languageEnglish
Pages (from-to)134-139
Journal / PublicationHypertension
Volume62
Issue number1
Publication statusPublished - Jul 2013
Externally publishedYes

Abstract

The small conductance and intermediate conductance Ca2+- activated K+ channels are known to be involved in the endothelium-dependent hyperpolarization. Ca2+ entry into endothelial cells stimulates these channels, causing membrane hyperpolarization in endothelial cells and underlying smooth muscle cells. In the present study, with the use of coimmunoprecipitation and double immunolabeling methods, we demonstrated a physical interaction of transient receptor potential vanilloid 4 (TRPV4) with KCa2.3 in rat mesenteric artery endothelial cells. Acetylcholine and 4α-PDD mainly acted through TRPV4-KCa2.3 pathway to induce smooth muscle hyperpolarization and vascular relaxation. KCa3.1 was also involved in the process but at a much lesser degree than that of KCa2.3. Stimulating TRPV4-KCa2.3 signaling pathway also increased local blood flow in mesenteric beds and reduced systemic blood pressure in anesthetized rats. In streptozotocin-induced diabetic rats, the expression levels of TRPV4 and KCa2.3 were reduced, which could be an underlying reason for the dysfunction of endothelium-dependent hyperpolarization in these animals. These results demonstrated an important physiological and pathological role of TRPV4-KCa2.3 signaling pathway in vascular endothelial cells. © 2013 American Heart Association, Inc.

Research Area(s)

  • endothelial cells, endothelium-derived hyperpolarizing factor, TRPV4-K Ca2.3 association, vascular relaxation

Bibliographic Note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to lbscholars@cityu.edu.hk.

Citation Format(s)

Functional role of TRPV4-KCa2.3 signaling in vascular endothelial cells in Normal and streptozotocin-induced diabetic rats. / Ma, Xin; Du, Juan; Zhang, Peng et al.
In: Hypertension, Vol. 62, No. 1, 07.2013, p. 134-139.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review