Functional neurological restoration of amputated peripheral nerve using biohybrid regenerative bioelectronics

Amy E. Rochford; Alejandro Carnicer-Lombarte; Malak Kawan; Amy Jin; Sam Hilton; Vincenzo F. Curto; Alexandra L. Rutz; Thomas Moreau; Mark R.N. Kotter; George G. Malliaras; Damiano G. Barone

doi:10.1126/sciadv.add8162

Functional neurological restoration of amputated peripheral nerve using biohybrid regenerative bioelectronics

Amy E. Rochford (Co-first Author)
, Alejandro Carnicer-Lombarte (Co-first Author)
, Malak Kawan
, Amy Jin
, Sam Hilton
, Vincenzo F. Curto
, Alexandra L. Rutz
, Thomas Moreau
, Mark R.N. Kotter
, George G. Malliaras^*
, Damiano G. Barone^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

53 Citations (Scopus)

2 Downloads (CityUHK Scholars)

Abstract

The development of neural interfaces with superior biocompatibility and improved tissue integration is vital for treating and restoring neurological functions in the nervous system. A critical factor is to increase the resolution for mapping neuronal inputs onto implants. For this purpose, we have developed a new category of neural interface comprising induced pluripotent stem cell (iPSC)-derived myocytes as biological targets for peripheral nerve inputs that are grafted onto a flexible electrode arrays. We show long-term survival and functional integration of a biohybrid device carrying human iPSC-derived cells with the forearm nerve bundle of freely moving rats, following 4 weeks of implantation. By improving the tissue-electronics interface with an intermediate cell layer, we have demonstrated enhanced resolution and electrical recording in vivo as a first step toward restorative therapies using regenerative bioelectronics. © 2023 American Association for the Advancement of Science. All rights reserved.

Original language	English
Article number	eadd8162
Journal	Science Advances
Volume	9
Issue number	12
Online published	22 Mar 2023
DOIs	https://doi.org/10.1126/sciadv.add8162
Publication status	Published - 24 Mar 2023
Externally published	Yes

Funding

.E.R., S.H.,and G.G.M. acknowledge funding from Engineering and Physical Sciences Research Council(EPSRC) (EP/S009000/1 and DTP program); A.C.-L. acknowledges funding from Wellcome Trustfor a Junior Interdisciplinary Fellowship and funding from the University of CambridgeBorysiewicz Interdisciplinary Fellowship program; V.F.C. and G.G.M. acknowledge funding fromEuropean Union’s Horizon 2020 Research and Innovation Programme under grant agreementno. 732032 (BrainCom); A.L.R. acknowledges funding from Whitaker International ScholarsProgram and the European Commission Horizon 2020 (Marie Sklodowska-Curie Fellowship797506); T.M. acknowledges support from bit.bio RnD department; M.R.N.K. acknowledgessupport by the Cambridge NIHR Brain Injury MedTech Cooperative and is funded by NIHRClinician Scientist Award CS-2015-15-023; and D.G.B. is supported by Health Education Englandand the National Institute for Health Research HEE/NIHR ICA Program Clinical Lectureship (CL-2019-14-004). The devices were assembled in the laboratory for prototyping softneuroprosthetic technologies, funded by the Sir Jules Thorn charitable trust (233838).

Publisher's Copyright Statement

This full text is made available under CC-BY 4.0. https://creativecommons.org/licenses/by/4.0/

Policy Impact

Cited in Policy Documents

Access Output

10.1126/sciadv.add8162

408119774.pdfFinal Published version, 1.22 MBLicence: CC BY

Other Access Options

Check CityUHK Library

Permanent Link

Right click to copy link

Cite this

@article{8b4fb9687db54eb882899bc860d8df0a,

title = "Functional neurological restoration of amputated peripheral nerve using biohybrid regenerative bioelectronics",

abstract = "The development of neural interfaces with superior biocompatibility and improved tissue integration is vital for treating and restoring neurological functions in the nervous system. A critical factor is to increase the resolution for mapping neuronal inputs onto implants. For this purpose, we have developed a new category of neural interface comprising induced pluripotent stem cell (iPSC)-derived myocytes as biological targets for peripheral nerve inputs that are grafted onto a flexible electrode arrays. We show long-term survival and functional integration of a biohybrid device carrying human iPSC-derived cells with the forearm nerve bundle of freely moving rats, following 4 weeks of implantation. By improving the tissue-electronics interface with an intermediate cell layer, we have demonstrated enhanced resolution and electrical recording in vivo as a first step toward restorative therapies using regenerative bioelectronics. {\textcopyright} 2023 American Association for the Advancement of Science. All rights reserved.",

author = "Rochford, \{Amy E.\} and Alejandro Carnicer-Lombarte and Malak Kawan and Amy Jin and Sam Hilton and Curto, \{Vincenzo F.\} and Rutz, \{Alexandra L.\} and Thomas Moreau and Kotter, \{Mark R.N.\} and Malliaras, \{George G.\} and Barone, \{Damiano G.\}",

year = "2023",

month = mar,

day = "24",

doi = "10.1126/sciadv.add8162",

language = "English",

volume = "9",

journal = "Science Advances",

issn = "2375-2548",

publisher = "American Association for the Advancement of Science",

number = "12",

}

Functional neurological restoration of amputated peripheral nerve using biohybrid regenerative bioelectronics. / Rochford, Amy E. (Co-first Author); Carnicer-Lombarte, Alejandro (Co-first Author); Kawan, Malak et al.
In: Science Advances, Vol. 9, No. 12, eadd8162, 24.03.2023.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

TY - JOUR

T1 - Functional neurological restoration of amputated peripheral nerve using biohybrid regenerative bioelectronics

AU - Rochford, Amy E.

AU - Carnicer-Lombarte, Alejandro

AU - Kawan, Malak

AU - Jin, Amy

AU - Hilton, Sam

AU - Curto, Vincenzo F.

AU - Rutz, Alexandra L.

AU - Moreau, Thomas

AU - Kotter, Mark R.N.

AU - Malliaras, George G.

AU - Barone, Damiano G.

PY - 2023/3/24

Y1 - 2023/3/24

N2 - The development of neural interfaces with superior biocompatibility and improved tissue integration is vital for treating and restoring neurological functions in the nervous system. A critical factor is to increase the resolution for mapping neuronal inputs onto implants. For this purpose, we have developed a new category of neural interface comprising induced pluripotent stem cell (iPSC)-derived myocytes as biological targets for peripheral nerve inputs that are grafted onto a flexible electrode arrays. We show long-term survival and functional integration of a biohybrid device carrying human iPSC-derived cells with the forearm nerve bundle of freely moving rats, following 4 weeks of implantation. By improving the tissue-electronics interface with an intermediate cell layer, we have demonstrated enhanced resolution and electrical recording in vivo as a first step toward restorative therapies using regenerative bioelectronics. © 2023 American Association for the Advancement of Science. All rights reserved.

AB - The development of neural interfaces with superior biocompatibility and improved tissue integration is vital for treating and restoring neurological functions in the nervous system. A critical factor is to increase the resolution for mapping neuronal inputs onto implants. For this purpose, we have developed a new category of neural interface comprising induced pluripotent stem cell (iPSC)-derived myocytes as biological targets for peripheral nerve inputs that are grafted onto a flexible electrode arrays. We show long-term survival and functional integration of a biohybrid device carrying human iPSC-derived cells with the forearm nerve bundle of freely moving rats, following 4 weeks of implantation. By improving the tissue-electronics interface with an intermediate cell layer, we have demonstrated enhanced resolution and electrical recording in vivo as a first step toward restorative therapies using regenerative bioelectronics. © 2023 American Association for the Advancement of Science. All rights reserved.

UR - https://www.scopus.com/pages/publications/85150768163#tab=impact

UR - https://app-overton-io.ezproxy.cityu.edu.hk/articles.php?query=10.1126/sciadv.add8162

UR - https://www.scopus.com/pages/publications/85150768163

U2 - 10.1126/sciadv.add8162

DO - 10.1126/sciadv.add8162

M3 - RGC 21 - Publication in refereed journal

C2 - 36947608

SN - 2375-2548

VL - 9

JO - Science Advances

JF - Science Advances

IS - 12

M1 - eadd8162

ER -

Functional neurological restoration of amputated peripheral nerve using biohybrid regenerative bioelectronics

Abstract

Funding

Publisher's Copyright Statement

Policy Impact

Access Output

Other Access Options

Permanent Link

Other Files and Links

Fingerprint

Cite this