Functional consortium for hydrogen production from cellobiose: Concentration-to-extinction approach

Sunil S. Adav; Duu-Jong Lee; Aijie Wang; Nanqi Ren

doi:10.1016/j.biortech.2008.12.014

Functional consortium for hydrogen production from cellobiose: Concentration-to-extinction approach

Sunil S. Adav
, Duu-Jong Lee
, Aijie Wang
, Nanqi Ren

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

50 Citations (Scopus)

Abstract

A functional bacterial consortium that can effectively hydrolyze cellobiose and produce bio-hydrogen was isolated by a concentration-to-extinction approach. The sludge from a cattle feedlot manure composting plant was incubated with 2.5-20 g l^-1 cellobiose at 35 °C and pH 6.0. The microbial diversity of serially concentrated suspensions significantly decreased following increasing cellobiose concentration, finally leaving only two viable strains, Clostridium butyricum strain W4 and Enterococcus saccharolyticus strain. This consortium has a maximum specific hydrogen production rate of 2.19 mol H₂ mol hexose^-1 at 5 g l^-1 cellobiose. The metabolic pathways shifted from ethanol-type to acetate-butyrate type as cellobiose concentration increased from 2.5 to >7 g l^-1. The concentration-to-extinction approach is effective for isolating functional consortium from natural microflora. In this case the functional strains of interest are more tolerant to the increased loadings of substrates than the non-functional strains. © 2008 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	2546-2550
Journal	Bioresource Technology
Volume	100
Issue number	9
DOIs	https://doi.org/10.1016/j.biortech.2008.12.014
Publication status	Published - May 2009
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy
SDG 12 Responsible Consumption and Production

Research Keywords

Cellobiose
Functional consortia
Hydrogen
Hydrolysis

Access Output

10.1016/j.biortech.2008.12.014

Other Access Options

Check CityUHK Library

Permanent Link

Right click to copy link

Cite this

@article{8ecbeb6cc9ce40f5a757af5572db9355,

title = "Functional consortium for hydrogen production from cellobiose: Concentration-to-extinction approach",

abstract = "A functional bacterial consortium that can effectively hydrolyze cellobiose and produce bio-hydrogen was isolated by a concentration-to-extinction approach. The sludge from a cattle feedlot manure composting plant was incubated with 2.5-20 g l-1 cellobiose at 35 °C and pH 6.0. The microbial diversity of serially concentrated suspensions significantly decreased following increasing cellobiose concentration, finally leaving only two viable strains, Clostridium butyricum strain W4 and Enterococcus saccharolyticus strain. This consortium has a maximum specific hydrogen production rate of 2.19 mol H2 mol hexose-1 at 5 g l-1 cellobiose. The metabolic pathways shifted from ethanol-type to acetate-butyrate type as cellobiose concentration increased from 2.5 to >7 g l-1. The concentration-to-extinction approach is effective for isolating functional consortium from natural microflora. In this case the functional strains of interest are more tolerant to the increased loadings of substrates than the non-functional strains. {\textcopyright} 2008 Elsevier Ltd. All rights reserved.",

keywords = "Cellobiose, Functional consortia, Hydrogen, Hydrolysis",

author = "Adav, \{Sunil S.\} and Duu-Jong Lee and Aijie Wang and Nanqi Ren",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected]. ",

year = "2009",

month = may,

doi = "10.1016/j.biortech.2008.12.014",

language = "English",

volume = "100",

pages = "2546--2550",

journal = "Bioresource Technology",

issn = "0960-8524",

publisher = "Elsevier Ltd.",

number = "9",

}

TY - JOUR

T1 - Functional consortium for hydrogen production from cellobiose

T2 - Concentration-to-extinction approach

AU - Adav, Sunil S.

AU - Lee, Duu-Jong

AU - Wang, Aijie

AU - Ren, Nanqi

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2009/5

Y1 - 2009/5

N2 - A functional bacterial consortium that can effectively hydrolyze cellobiose and produce bio-hydrogen was isolated by a concentration-to-extinction approach. The sludge from a cattle feedlot manure composting plant was incubated with 2.5-20 g l-1 cellobiose at 35 °C and pH 6.0. The microbial diversity of serially concentrated suspensions significantly decreased following increasing cellobiose concentration, finally leaving only two viable strains, Clostridium butyricum strain W4 and Enterococcus saccharolyticus strain. This consortium has a maximum specific hydrogen production rate of 2.19 mol H2 mol hexose-1 at 5 g l-1 cellobiose. The metabolic pathways shifted from ethanol-type to acetate-butyrate type as cellobiose concentration increased from 2.5 to >7 g l-1. The concentration-to-extinction approach is effective for isolating functional consortium from natural microflora. In this case the functional strains of interest are more tolerant to the increased loadings of substrates than the non-functional strains. © 2008 Elsevier Ltd. All rights reserved.

AB - A functional bacterial consortium that can effectively hydrolyze cellobiose and produce bio-hydrogen was isolated by a concentration-to-extinction approach. The sludge from a cattle feedlot manure composting plant was incubated with 2.5-20 g l-1 cellobiose at 35 °C and pH 6.0. The microbial diversity of serially concentrated suspensions significantly decreased following increasing cellobiose concentration, finally leaving only two viable strains, Clostridium butyricum strain W4 and Enterococcus saccharolyticus strain. This consortium has a maximum specific hydrogen production rate of 2.19 mol H2 mol hexose-1 at 5 g l-1 cellobiose. The metabolic pathways shifted from ethanol-type to acetate-butyrate type as cellobiose concentration increased from 2.5 to >7 g l-1. The concentration-to-extinction approach is effective for isolating functional consortium from natural microflora. In this case the functional strains of interest are more tolerant to the increased loadings of substrates than the non-functional strains. © 2008 Elsevier Ltd. All rights reserved.

KW - Cellobiose

KW - Functional consortia

KW - Hydrogen

KW - Hydrolysis

UR - https://www.scopus.com/pages/publications/59649127998

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-59649127998&origin=recordpage

U2 - 10.1016/j.biortech.2008.12.014

DO - 10.1016/j.biortech.2008.12.014

M3 - RGC 21 - Publication in refereed journal

C2 - 19138842

SN - 0960-8524

VL - 100

SP - 2546

EP - 2550

JO - Bioresource Technology

JF - Bioresource Technology

IS - 9

ER -

Functional consortium for hydrogen production from cellobiose: Concentration-to-extinction approach

Abstract

Bibliographical note

UN SDGs

Research Keywords

Access Output

Other Access Options

Permanent Link

Other Files and Links

Fingerprint

Cite this