Functional and phylogenetic analysis of TetX variants to design a new classification system
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
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Detail(s)
Original language | English |
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Article number | 522 |
Journal / Publication | Communications Biology |
Volume | 5 |
Online published | 31 May 2022 |
Publication status | Published - 2022 |
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DOI | DOI |
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Attachment(s) | Documents
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Link to Scopus | https://www.scopus.com/record/display.uri?eid=2-s2.0-85130983152&origin=recordpage |
Permanent Link | https://scholars.cityu.edu.hk/en/publications/publication(e6c8e31d-6cdd-4fb9-9cce-1dcd1d3dcb99).html |
Abstract
Recently, many TetX variants such as Tet(X3 similar to 14) were reported to confer resistance to tigecycline which is a last-resort antibiotic used to treat infections caused by multidrug-resistant bacteria. In this study, we identified essential residues including 329, 339, 340, 350, and 351 in TetX variants that mediated the evolution of the tigecycline-inactive Tet(X2) enzyme to the active forms of Tet(X3) and Tet(X4). Based on their amino acid sequences and functional features, we classified TetX variants into TetX-A class, TetX-B class and TetX-C class. We further found that TetX-A class variants originated from Bacteroidetes, with some variants further evolving to TetX-C class and acquired by Enterobacteriaceae. On the other hand, our data showed that some variants genes belonging to TetX-A class evolved directly to TetX-B class, which was further transmitted to Acinetobacter spp. This new classification system may facilitate better clinical management of patients infected by TetX-producing strains.
Research Area(s)
- TIGECYCLINE RESISTANCE GENE, TETRACYCLINE RESISTANCE, ANTIBIOTICS, MECHANISM, PROTEUS, TET(X6), SEARCH
Citation Format(s)
Functional and phylogenetic analysis of TetX variants to design a new classification system. / Cheng, Qipeng; Cheung, Yanchu; Liu, Chenyu et al.
In: Communications Biology, Vol. 5, 522, 2022.
In: Communications Biology, Vol. 5, 522, 2022.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
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