Fully motorized optical-resolution photoacoustic microscopy

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Lei Li
  • Chenghung Yeh
  • Song Hu
  • Brian T. Soetikno
  • Ruimin Chen
  • Qifa Zhou
  • K. Kirk Shung
  • Konstantin I. Maslov
  • Lihong V. Wang

Detail(s)

Original languageEnglish
Pages (from-to)2117-2120
Journal / PublicationOptics Letters
Volume39
Issue number7
Publication statusPublished - 1 Apr 2014
Externally publishedYes

Abstract

We have developed fully motorized optical-resolution photoacoustic microscopy (OR-PAM), which integrates five complementary scanning modes and simultaneously provides a high imaging speed and a wide field of view (FOV) with 2.6 μm lateral resolution. With one-dimensional (1D) motion-mode mechanical scanning, we measured the blood flow through a cross section of a blood vessel in vivo. With two-dimensional (2D) optical scanning at a laser repetition rate of 40 kHz, we achieved a 2 kHz B-scan rate over a range of 50 μm with 20 A-lines and 50 Hz volumetric-scan rate over a FOV of 50 μm × 50 μm with 400 A-lines, which enabled real-time tracking of cellular dynamics in vivo. With synchronized 1D optical and 2D mechanical hybrid scanning, we imaged a 10 mm × 8 mm FOV within three minutes, which is 20 times faster than the conventional mechanical scan in our second-generation OR-PAM. With three-dimensional mechanical contour scanning, we maintained the optimal signal-to-noise ratio and spatial resolution of OR-PAM while imaging objects with uneven surfaces, which is essential for quantitative studies. © 2014 Optical Society of America.

Citation Format(s)

Fully motorized optical-resolution photoacoustic microscopy. / Li, Lei; Yeh, Chenghung; Hu, Song et al.
In: Optics Letters, Vol. 39, No. 7, 01.04.2014, p. 2117-2120.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review