Framework nucleic acids as programmable carrier for transdermal drug delivery

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Christian Wiraja
  • Ying Zhu
  • Daniel Chin Shiuan Lio
  • David C. Yeo
  • Mo Xie
  • Weina Fang
  • Qian Li
  • Mengjia Zheng
  • Maurice Van Steensel
  • Lihua Wang
  • Chunhai Fan

Detail(s)

Original languageEnglish
Article number1147
Journal / PublicationNature Communications
Volume10
Online published8 Mar 2019
Publication statusPublished - 2019
Externally publishedYes

Abstract

DNA nanostructures are promising drug carriers with their intrinsic biocompatibility, uniformity and versatility. However, rapid serum disintegration leads to low bioavailability at targeted sites following systemic administration, hindering their biomedical applications. Here we demonstrate transdermal delivery of framework nucleic acids (FNAs) through topical applications. By designing FNAs with distinct shapes and sizes, we interrogate their penetration on mice and human skin explant. Skin histology reveals size-dependent penetration, with FNAs ≤75 nm effectively reaching dermis layer. 17 nm-tetrahedral FNAs show greatest penetration to 350 µm from skin periphery. Importantly, structural integrity is maintained during the skin penetration. Employing a mouse melanoma model, topical application of doxorubicin-loaded FNAs accommodates ≥2-fold improvement in drug accumulation and tumor inhibition relative to topically-applied free doxorubicin, or doxorubicin loaded in liposomes and polymeric nanoparticles. Programmable penetration with minimal systemic biodistribution underlines FNA potential as localized transdermal drug delivery carriers.

Citation Format(s)

Framework nucleic acids as programmable carrier for transdermal drug delivery. / Wiraja, Christian; Zhu, Ying; Lio, Daniel Chin Shiuan; Yeo, David C.; Xie, Mo; Fang, Weina; Li, Qian; Zheng, Mengjia; Van Steensel, Maurice; Wang, Lihua; Fan, Chunhai; Xu, Chenjie.

In: Nature Communications, Vol. 10, 1147, 2019.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal