Fluorescent Poly(glycerol-co-sebacate) Acrylate Nanoparticles for Stem Cell Labeling and Longitudinal Tracking

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Lifeng Wang
  • Keming Xu
  • Xiaochun Hou
  • Yiyuan Han
  • Shiying Liu
  • Christian Wiraja
  • Cangjie Yang
  • Jun Yang
  • Mingfeng Wang
  • Xiaochen Dong
  • Wei Huang

Detail(s)

Original languageEnglish
Pages (from-to)9528-9538
Journal / PublicationACS Applied Materials and Interfaces
Volume9
Issue number11
Online published1 Mar 2017
Publication statusPublished - 22 Mar 2017
Externally publishedYes

Abstract

The stable presence of fluorophores within the biocompatible and biodegradable elastomer poly(glycerol-co-sebacate) acrylate (PGSA) is critical for monitoring the transplantation, performance, and degradation of the polymers in vivo. However, current methods such as physically entrapping the fluorophores in the polymer matrix or providing a fluorescent coating suffer from rapid leakage of fluorophores. Covalent conjugation of fluorophores with the polymers and the subsequent core-cross-linking are proposed here to address this challenge. Taking rhodamine as the model dye and PGSA nanoparticles (NPs) as the model platform, we successfully showed that the synthesized rhodamine-conjugated PGSA (PGSAR) NPs only released less than 30% rhodamine at day 28, whereas complete release of dye occurred for rhodamine-encapsulated PGSA (PGSA-p-R) NPs at day 7 and 57.49% rhodamine was released out for the un-cross-linked PGSAR NPs at day 28. More excitingly, PGSAR NPs showed a strong quantum yield enhancement (26.24-fold) of the fluorophores, which was due to the hydrophobic environment within PGSAR NPs and the restricted rotation of (6-diethylamino-3H-xanthen-3-ylidene) diethyl group in rhodamine after the conjugation and core-cross-linking. The stable presence of dye in the NPs and enhanced fluorescence allowed a longitudinal tracking of stem cells both in vitro and in vivo for at least 28 days.

Research Area(s)

  • longitudinal tracking, nanoparticles, poly(glycerol-co-sebacate) acrylate, rhodamine, stem cell labeling

Citation Format(s)

Fluorescent Poly(glycerol-co-sebacate) Acrylate Nanoparticles for Stem Cell Labeling and Longitudinal Tracking. / Wang, Lifeng; Xu, Keming; Hou, Xiaochun; Han, Yiyuan; Liu, Shiying; Wiraja, Christian; Yang, Cangjie; Yang, Jun; Wang, Mingfeng; Dong, Xiaochen; Huang, Wei; Xu, Chenjie.

In: ACS Applied Materials and Interfaces, Vol. 9, No. 11, 22.03.2017, p. 9528-9538.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review