Fluorecent staining for study of extracellular polymeric substances in membrane biofouling layers

Ming-Yuan Chen; Duu-Jong Lee; Z. Yang; X. F. Peng; J. Y. Lai

doi:10.1021/es0612955

Fluorecent staining for study of extracellular polymeric substances in membrane biofouling layers

Ming-Yuan Chen
, Duu-Jong Lee
, Z. Yang
, X. F. Peng
, J. Y. Lai

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

155 Citations (Scopus)

Abstract

Membrane biofouling by microbial products adversely impacts the feasibility of adopting membrane bioreactors (MBRs) for treating wastewater. The fouling layer structure determines the pressure drop across the fouling layer. Three-dimensional distributions of nucleic acids, proteins, α-D- GLUCOPYRANOSE POLYSACCHARIDES, AND B-D-glucopyranose polysaccharides in the fouling layer formed on a mixed cellulose ester membrane were generated utilizing a quadruple staining protocol combined with confocal laser scanning microscopy (CLSM). For the first time, this study constructed a three-dimensional volumetric grids model representing the fouling layer structure on the basis of a series of CLSM images. Quantitative structural information about the fouling layer was extracted from the CLSM images. © 2006 American Chemical Society.

Original language	English
Pages (from-to)	6642-6646
Journal	Environmental Science and Technology
Volume	40
Issue number	21
DOIs	https://doi.org/10.1021/es0612955
Publication status	Published - 1 Nov 2006
Externally published	Yes

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Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 6 Clean Water and Sanitation

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title = "Fluorecent staining for study of extracellular polymeric substances in membrane biofouling layers",

abstract = "Membrane biofouling by microbial products adversely impacts the feasibility of adopting membrane bioreactors (MBRs) for treating wastewater. The fouling layer structure determines the pressure drop across the fouling layer. Three-dimensional distributions of nucleic acids, proteins, α-D- GLUCOPYRANOSE POLYSACCHARIDES, AND B-D-glucopyranose polysaccharides in the fouling layer formed on a mixed cellulose ester membrane were generated utilizing a quadruple staining protocol combined with confocal laser scanning microscopy (CLSM). For the first time, this study constructed a three-dimensional volumetric grids model representing the fouling layer structure on the basis of a series of CLSM images. Quantitative structural information about the fouling layer was extracted from the CLSM images. {\textcopyright} 2006 American Chemical Society.",

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TY - JOUR

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AU - Chen, Ming-Yuan

AU - Lee, Duu-Jong

AU - Yang, Z.

AU - Peng, X. F.

AU - Lai, J. Y.

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

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N2 - Membrane biofouling by microbial products adversely impacts the feasibility of adopting membrane bioreactors (MBRs) for treating wastewater. The fouling layer structure determines the pressure drop across the fouling layer. Three-dimensional distributions of nucleic acids, proteins, α-D- GLUCOPYRANOSE POLYSACCHARIDES, AND B-D-glucopyranose polysaccharides in the fouling layer formed on a mixed cellulose ester membrane were generated utilizing a quadruple staining protocol combined with confocal laser scanning microscopy (CLSM). For the first time, this study constructed a three-dimensional volumetric grids model representing the fouling layer structure on the basis of a series of CLSM images. Quantitative structural information about the fouling layer was extracted from the CLSM images. © 2006 American Chemical Society.

AB - Membrane biofouling by microbial products adversely impacts the feasibility of adopting membrane bioreactors (MBRs) for treating wastewater. The fouling layer structure determines the pressure drop across the fouling layer. Three-dimensional distributions of nucleic acids, proteins, α-D- GLUCOPYRANOSE POLYSACCHARIDES, AND B-D-glucopyranose polysaccharides in the fouling layer formed on a mixed cellulose ester membrane were generated utilizing a quadruple staining protocol combined with confocal laser scanning microscopy (CLSM). For the first time, this study constructed a three-dimensional volumetric grids model representing the fouling layer structure on the basis of a series of CLSM images. Quantitative structural information about the fouling layer was extracted from the CLSM images. © 2006 American Chemical Society.

UR - https://www.scopus.com/pages/publications/33750867648

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U2 - 10.1021/es0612955

DO - 10.1021/es0612955

M3 - RGC 21 - Publication in refereed journal

C2 - 17144290

SN - 0013-936X

VL - 40

SP - 6642

EP - 6646

JO - Environmental Science and Technology

JF - Environmental Science and Technology

IS - 21

ER -

Fluorecent staining for study of extracellular polymeric substances in membrane biofouling layers

Abstract

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