Flow-through sampling for electrophoresis-based microfluidic chips using hydrodynamic pumping

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Yi-Hung Lin
  • Gwo-Bin Lee
  • Chun-Wei Li
  • Guan-Ruey Huang
  • Shu-Hui Chen

Detail(s)

Original languageEnglish
Pages (from-to)115-125
Journal / PublicationJournal of Chromatography A
Volume937
Issue number1-2
Online published17 Oct 2001
Publication statusPublished - 7 Dec 2001
Externally publishedYes

Abstract

This work presents a novel electrophoretic microchip design which is capable of directly coupling with flow-through analyzers for uninterrupted sampling. In this device, a 3 mm wide sampling channel (SC) was etched on quartz substrate to create the sample inlet and outlet and the 75 μm wide electrophoretic channels were also fabricated on the same substrate. Pressure was used to drive the sample flow through the external tube into the SC and the flow was then split into outlet and electrophoretic channels. A gating voltage was applied to the electrophoretic channel to control the sample loading for subsequent separations and inhibit the sample leakage. The minimum gating voltage required to inhibit the sample leakage depended on the solution buffer and increased with the hydrodynamic flow-rate. A fluorescent dye mixture containing Rhodamine B and Cy3 was introduced into the sample stream at either a continuous or discrete mode via an on-line injection valve and then separated and detected on the microchip using laser-induced fluorescence. For both modes, the relative standard deviation of migration time and peak intensity for consecutive injections was determined to be below 0.6 and 8%, respectively. Because the SC was kept floating, the external sampling equipment requires no electric connection. Therefore, such an electrophoresis-based microchip can be directly coupled with any pressure-driven flow analyzers without hardware modifications. To our best knowledge, this is something currently impossible for reported electrophoretic microchip designs. © 2001 Elsevier Science B.V. All rights reserved.

Research Area(s)

  • Chip technology, Instrumentation, Rhodamine B, Sample handling, Sodium carbonate, Sodium tetraborate

Citation Format(s)

Flow-through sampling for electrophoresis-based microfluidic chips using hydrodynamic pumping. / Lin, Yi-Hung; Lee, Gwo-Bin; Li, Chun-Wei; Huang, Guan-Ruey; Chen, Shu-Hui.

In: Journal of Chromatography A, Vol. 937, No. 1-2, 07.12.2001, p. 115-125.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review