Five-wavelength optical-resolution photoacoustic microscopy of blood and lymphatic vessels

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

View graph of relations

Detail(s)

Original languageEnglish
Article number016002
Journal / PublicationAdvanced Photonics
Volume3
Issue number1
Online published4 Jan 2021
Publication statusPublished - Jan 2021

Link(s)

Abstract

Optical-resolution photoacoustic microscopy (OR-PAM) has been developed for anatomical, functional, and molecular imaging but usually requires multiple scanning for different contrasts. We present five-wavelength OR-PAM for simultaneous imaging of hemoglobin concentration, oxygen saturation, blood flow speed, and lymphatic vessels in single raster scanning. We develop a five-wavelength pulsed laser via stimulated Raman scattering. The five pulsed wavelengths, i.e., 532, 545, 558, 570, and 620/640 nm, are temporally separated by several hundreds of nanoseconds via different optical delays in fiber. Five photoacoustic images at these wavelengths are simultaneously acquired in a single scanning. The 532- and 620/640-nm wavelengths are used to image the blood vessels and dye-labeled lymphatic vessels. The blood flow speed is measured by a dual-pulse method. The oxygen saturation is calculated and compensated for by the Gruneisen-relaxation effect. In vivo imaging of hemoglobin concentration, oxygen saturation, blood flow speed, and lymphatic vessels is demonstrated in preclinical applications of cancer detection, lymphatic clearance monitoring, and functional brain imaging.

Research Area(s)

  • optical-resolution photoacoustic microscopy, stimulated Raman scattering, single-shot, multi-contrast microscopy, early cancer detection, MULTISPECTRAL OPTOACOUSTIC TOMOGRAPHY, INDOCYANINE GREEN, CANCER-CELLS, OXYGEN, LYMPHOGRAPHY, TUMORS, NODES, RATS

Download Statistics

No data available