Fish Capsules : A System for High-Throughput Screening of Combinatorial Drugs
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
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Detail(s)
Original language | English |
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Article number | 2104449 |
Journal / Publication | Advanced Science |
Volume | 9 |
Issue number | 9 |
Online published | 27 Jan 2022 |
Publication status | Published - 25 Mar 2022 |
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DOI | DOI |
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Attachment(s) | Documents
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Link to Scopus | https://www.scopus.com/record/display.uri?eid=2-s2.0-85123726896&origin=recordpage |
Permanent Link | https://scholars.cityu.edu.hk/en/publications/publication(8cfcbec6-6530-40fb-b162-603cdfe8886e).html |
Abstract
Large-scale screening of molecules heavily relies on phenotyping of small living organisms during preclinical development. However, deep profiling candidate therapeutics on whole animals typically requires laborious manipulations and anesthetic treatment using traditional techniques or automated tools. Here, a novel fish capsule system that combines automated zebrafish encapsulating technology and droplet microarray strategy for in vivo functional screening of mono/polytherapies is described. This platform enables automated, rapid zebrafish orientation and immobilization in agarose to generate large-scale fish capsules by using a microfluidic device. Based on the effect of discontinuous dewetting, the prompt trapping of fish capsules in the aqueous arrays is successfully demonstrate. This system provides the capability to integrate pharmaceutical treatments with real-time multispectral microscopic imaging in a simple, pipetting-free and highly parallel manner. Coupling with machine learning algorithms, a small library of compounds is screened and analyzed, and clues about how to exploit compound combinations as therapeutic candidates are obtained. It is believed that this proposed strategy can be readily applied to multiple fields and is especially useful in the exploration of combinatorial drugs with limited amounts of samples and resources to accelerate the identification of novel therapeutics for precision medicines.
Research Area(s)
- drug combination, high-throughput screening, microdroplet array, mi-crofluidics, zebrafish
Citation Format(s)
Fish Capsules: A System for High-Throughput Screening of Combinatorial Drugs. / Tang, Minghui; Duan, Xin; Yang, Anqi et al.
In: Advanced Science, Vol. 9, No. 9, 2104449, 25.03.2022.
In: Advanced Science, Vol. 9, No. 9, 2104449, 25.03.2022.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
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