TY - JOUR
T1 - Fine and ultrafine airborne PM influence inflammation response of young adults and toxicological responses in vitro
AU - Yang, Mo
AU - Jalava, Pasi
AU - Hakkarainen, Henri
AU - Roponen, Marjut
AU - Leskinen, Ari
AU - Komppula, Mika
AU - Dong, Guo-Ping
AU - Lao, Xiang-Qian
AU - Wu, Qi-Zhen
AU - Xu, Shu-Li
AU - Lin, Li-Zi
AU - Liu, Ru-Qing
AU - Hu, Li-Wen
AU - Yang, Bo-Yi
AU - Zeng, Xiao-Wen
AU - Dong, Guang-Hui
PY - 2022/8/25
Y1 - 2022/8/25
N2 - Little evidence is available regarding the impact of different sizes of inhaled particulate matter (PM) on inflammatory responses in healthy young adults in connection with toxicological responses. We conducted a five-time repeated measurement panel study on 88 healthy young college students in Guangzhou, China from December 2017 to January 2018. Blood samples were collected from each participant and tested for tumor necrosis factor alpha (TNF-α) levels every week for 5 consecutive weeks. Mass concentrations of ambient PM2.5, PM1, PM0.5 and number concentrations of ambient PM0.1 were measured. RAW 264.7 macrophages were exposed to PM (PM10–2.5, PM2.5–1, PM1–0.2, PM0.2) collected at the same time as the panel study. Cytotoxicity, oxidation and inflammatory parameters, cell cycle and genotoxicity were tested. Particles were characterized for their chemical composition. The trends of associations between PM2.5, PM1, PM0.5 and TNF-α level were consistent in lag 0 and 3 days, and the relative risk decreased as the particle size decreased. All the ambient air pollutants had the similar change trends in lag 1, 4 and 5 days. Similar results in RAW 264.7 macrophages were found; PM10–2.5 induced the greatest TNF-α and macrophage inflammatory protein 2 (MIP-2) productions and oxidative damage. PM1–0.2 and PM0.2 induced more significant dose-dependent increases of cell cycle and genotoxic response. In the component concentrations of PM samples, metal elements were PM10–2.5 > PM2.5–1 > PM0.2 ≥ PM1–0.2; ions and polycyclic aromatic hydrocarbons (PAHs) were PM0.2 > PM1–0.2 > PM2.5–1 > PM10–2.5. Our results suggested that exposure to all particle sizes was significantly associated with inflammation among healthy young adults and toxicological responses in RAW 264.7 macrophages. Different human and toxicological reactions caused by PM samples indicated the importance of investigating various particle sizes. © 2022 Elsevier B.V.
AB - Little evidence is available regarding the impact of different sizes of inhaled particulate matter (PM) on inflammatory responses in healthy young adults in connection with toxicological responses. We conducted a five-time repeated measurement panel study on 88 healthy young college students in Guangzhou, China from December 2017 to January 2018. Blood samples were collected from each participant and tested for tumor necrosis factor alpha (TNF-α) levels every week for 5 consecutive weeks. Mass concentrations of ambient PM2.5, PM1, PM0.5 and number concentrations of ambient PM0.1 were measured. RAW 264.7 macrophages were exposed to PM (PM10–2.5, PM2.5–1, PM1–0.2, PM0.2) collected at the same time as the panel study. Cytotoxicity, oxidation and inflammatory parameters, cell cycle and genotoxicity were tested. Particles were characterized for their chemical composition. The trends of associations between PM2.5, PM1, PM0.5 and TNF-α level were consistent in lag 0 and 3 days, and the relative risk decreased as the particle size decreased. All the ambient air pollutants had the similar change trends in lag 1, 4 and 5 days. Similar results in RAW 264.7 macrophages were found; PM10–2.5 induced the greatest TNF-α and macrophage inflammatory protein 2 (MIP-2) productions and oxidative damage. PM1–0.2 and PM0.2 induced more significant dose-dependent increases of cell cycle and genotoxic response. In the component concentrations of PM samples, metal elements were PM10–2.5 > PM2.5–1 > PM0.2 ≥ PM1–0.2; ions and polycyclic aromatic hydrocarbons (PAHs) were PM0.2 > PM1–0.2 > PM2.5–1 > PM10–2.5. Our results suggested that exposure to all particle sizes was significantly associated with inflammation among healthy young adults and toxicological responses in RAW 264.7 macrophages. Different human and toxicological reactions caused by PM samples indicated the importance of investigating various particle sizes. © 2022 Elsevier B.V.
KW - Airborne PM
KW - Inflammation response
KW - Toxicological responses
KW - Young adults
UR - http://www.scopus.com/inward/record.url?scp=85129744744&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85129744744&origin=recordpage
U2 - 10.1016/j.scitotenv.2022.155618
DO - 10.1016/j.scitotenv.2022.155618
M3 - RGC 21 - Publication in refereed journal
C2 - 35513150
SN - 0048-9697
VL - 836
JO - Science of the Total Environment
JF - Science of the Total Environment
M1 - 155618
ER -