Fermentative hydrogen production by Clostridium butyricum CGS5 using carbohydrate-rich microalgal biomass as feedstock

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Chien-Hung Liu
  • Chin-Yen Chang
  • Chieh-Lun Cheng
  • Duu-Jong Lee
  • Jo-Shu Chang

Detail(s)

Original languageEnglish
Pages (from-to)15458-15464
Journal / PublicationInternational Journal of Hydrogen Energy
Volume37
Issue number20
Publication statusPublished - Oct 2012
Externally publishedYes

Abstract

In this work, a carbohydrate-rich microalga, Chlorella vulgaris ESP6, was grown photoautotrophically to fix the CO 2. The resulting microalgal biomass was hydrolyzed by acid or alkaline/enzymatic treatment and was then used for biohydrogen production with Clostridium butyricum CGS5. The C. vulgaris biomass could be effectively hydrolyzed by acid pretreatment while similar hydrolysis efficiency was achieved by combination of alkaline pretreatment and enzymatic hydrolysis. The biomass of C. vulgaris ESP6 containing a carbohydrate content of 57% (dry weight basis) was efficiently hydrolyzed by acid treatment with 1.5% HCl, giving a reducing sugars (RS) yield of nearly 100%. C. butyricum CGS5 could utilize RS from C. vulgaris ESP6 biomass to produce hydrogen without any additional organic carbon sources. The optimal conditions for hydrogen production were 37 °C and a microalgal hydrolysate loading of 9 g RS/L with pH-controlled at 5.5. Under the optimal conditions, the cumulative H 2 production, H 2 production rate, and H 2 yield were 1476 ml/L, 246 ml/L/h, and 1.15 mol/mol RS, respectively. The results demonstrate that the C. vulgaris biomass has the potential to serve as effective feedstock for dark fermentative H 2 production. © 2012, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.

Research Area(s)

  • Biohydrogen, Chlorella vulgaris, Clostridium butyricum, CO 2 fixation, Microalgae-based carbohydrates

Bibliographic Note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Citation Format(s)

Fermentative hydrogen production by Clostridium butyricum CGS5 using carbohydrate-rich microalgal biomass as feedstock. / Liu, Chien-Hung; Chang, Chin-Yen; Cheng, Chieh-Lun et al.
In: International Journal of Hydrogen Energy, Vol. 37, No. 20, 10.2012, p. 15458-15464.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review