Femto-second Laser-based free writing of 3D protein microstructures and micropatterns with Sub-micrometer features : A study on voxels, porosity, and cytocompatibility

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

23 Scopus Citations
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Author(s)

  • Barbara Pui Chan
  • Jiao Ni Ma
  • Jin Ye Xu
  • Chuen Wai Li
  • Jin Ping Cheng

Detail(s)

Original languageEnglish
Pages (from-to)277-294
Journal / PublicationAdvanced Functional Materials
Volume24
Issue number3
Online published16 Jul 2013
Publication statusPublished - 22 Jan 2014

Abstract

Femto-second laser-based free-writing of complex protein microstructures and micropatterns, with sub-micrometer features and controllability over voxel dimension, morphology, and porosity, is reported. Protein voxels including lines, spots, and micropillars are fabricated. Laser power, exposure time, z-position, protein and photosensitizer concentrations, but not scanning speed, are important controlling parameters. A lateral fabrication resolution of ≈200 nm is demonstrated in 2D line voxels. 3D spot voxels are ellipsoids with 400 nm lateral and 1.5 μm axial dimensions. An ascending z-stack scanning method to verify the theoretical axial optical resolution, delineate and enhance the axial fabrication resolution of 3D structures, including square prism and cylinder micropillars, is also reported. The micropillar array presents a simple "write-and-seed" and table platform for cell niche studies. Fibroblasts attach to, grow on, and express adhesion to molecules on micropillar arrays without the need of matrix coating. They exhibit a more "3D" morphology comparing with that in 2D monolayer cultures and physiological functions such as matrix deposition. This work presents an important milestone in engineering complex protein microstructures and micropatterns with sub-micrometer topological features to mimic the native matrix niche for cell-matrix interaction studies. Multiphoton-based free-writing of 3D complex protein microstructures and micropatterns, with sub-micrometer features and controllability over voxel dimension, morphology, and porosity, is reported. Protein micropillar array presents a simple "write-and-seed" platform for cell niche studies. Fibroblasts attach to, grow on, express adhesion molecules and deposit extracellular matrices on these arrays without matrix coating. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Research Area(s)

  • femto-second laser, multiphoton, photochemical crosslinking, protein voxel, sub-micrometer fabrication

Citation Format(s)

Femto-second Laser-based free writing of 3D protein microstructures and micropatterns with Sub-micrometer features : A study on voxels, porosity, and cytocompatibility. / Chan, Barbara Pui; Ma, Jiao Ni; Xu, Jin Ye; Li, Chuen Wai; Cheng, Jin Ping; Cheng, Shuk Han.

In: Advanced Functional Materials, Vol. 24, No. 3, 22.01.2014, p. 277-294.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal