TY - JOUR
T1 - Fast 3-D temporal focusing microscopy using an electrically tunable lens
AU - Jiang, Jun
AU - Zhang, Dapeng
AU - Walker, Steven
AU - Gu, Chenglin
AU - Ke, Ya
AU - Yung, Wing Ho
AU - Chen, Shih-Chi
N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].
PY - 2015
Y1 - 2015
N2 - In this paper, we present a 3-D temporal focusing microscope based on an electrically tunable lens (ETL) and a femtosecond regenerative laser amplifier. The focus-tunable lens provides a fast and compact way to perform non-mechanical z-scanning and resolves the blurry image issue compared with GVD-based z-scanning methods. The optical performance of the temporal focusing system, including z-scanning characteristics, the associated the magnification variation, and the lateral and axial resolution, has been studied and characterized using calibrated Rhodamine-6G thin film sample, fluorescent beads, and pollen samples. Lastly, we demonstrate the optical cross-sectioning and z-scanning capability with an in vivo experiment, where Ca2+ imaging of neurons in GaCamp6 labeled zebrafish was performed. © 2015 Optical Society of America.
AB - In this paper, we present a 3-D temporal focusing microscope based on an electrically tunable lens (ETL) and a femtosecond regenerative laser amplifier. The focus-tunable lens provides a fast and compact way to perform non-mechanical z-scanning and resolves the blurry image issue compared with GVD-based z-scanning methods. The optical performance of the temporal focusing system, including z-scanning characteristics, the associated the magnification variation, and the lateral and axial resolution, has been studied and characterized using calibrated Rhodamine-6G thin film sample, fluorescent beads, and pollen samples. Lastly, we demonstrate the optical cross-sectioning and z-scanning capability with an in vivo experiment, where Ca2+ imaging of neurons in GaCamp6 labeled zebrafish was performed. © 2015 Optical Society of America.
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U2 - 10.1364/OE.23.024362
DO - 10.1364/OE.23.024362
M3 - RGC 21 - Publication in refereed journal
SN - 1094-4087
VL - 23
SP - 24362
EP - 24368
JO - Optics Express
JF - Optics Express
IS - 19
ER -