Facile construction of a DNA tetrahedron in unconventional ladder-like arrangements at room temperature

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

1 Scopus Citations
View graph of relations

Detail(s)

Original languageEnglish
Pages (from-to)1240-1248
Journal / PublicationNanoscale Advances
Volume1
Issue number3
Online published27 Dec 2018
Publication statusPublished - 1 Mar 2019

Link(s)

Abstract

A DNA tetrahedron as the most classical and simplest three-dimensional DNA nanostructure has been widely utilized in biomedicine and biosensing. However, the existing assembly approaches usually require harsh thermal annealing conditions, involve the formation of unwanted by-products, and have poor size control. Herein, a facile strategy to fabricate a discrete DNA tetrahedron as a single, thermodynamically stable product in a quantitative yield at room temperature is reported. This system does not require a DNA trigger or thermal annealing treatment to initiate self-assembly. This DNA tetrahedron was made of three chemically ligated triangular-shaped DNAs in unconventional ladder-like arrangements, with measured heights of ∼4.16 ± 0.04 nm, showing extra protections for enzymatic degradation in biological environment. They show substantial cellular uptake in different cell lines via temperature, energy-dependent and clathrin-mediated endocytosis pathways. These characteristics allow our DNA tetrahedron to be used as vehicles for the delivery of very small and temperature-sensitive cargos. This novel assembly strategy developed for DNA tetrahedra could potentially be extended to other highly complex polyhedra; this indicated its generalizability.

Research Area(s)

Citation Format(s)

Facile construction of a DNA tetrahedron in unconventional ladder-like arrangements at room temperature. / Dai, Ziwen; Leung, Hoi Man; Gao, Qi; Wang, Fei; Wong, Sze Wing; Liu, Ling Sum; Au, Yu Ju; Lai, King Wai Chiu; Lo, Pik Kwan.

In: Nanoscale Advances, Vol. 1, No. 3, 01.03.2019, p. 1240-1248.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

Download Statistics

No data available