Fabrication and characterization of monodisperse PLGA-alginate core-shell microspheres with monodisperse size and homogeneous shells for controlled drug release

Jun Wu; Tiantian Kong; Kelvin Wai Kwok Yeung; Ho Cheung Shum; Kenneth Man Chee Cheung; Liqiu Wang; Michael Kai Tsun To

doi:10.1016/j.actbio.2013.03.022

Fabrication and characterization of monodisperse PLGA-alginate core-shell microspheres with monodisperse size and homogeneous shells for controlled drug release

Jun Wu
, Tiantian Kong
, Kelvin Wai Kwok Yeung
, Ho Cheung Shum
, Kenneth Man Chee Cheung
, Liqiu Wang
, Michael Kai Tsun To

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

177 Citations (Scopus)

Abstract

Monodisperse PLGA-alginate core-shell microspheres with controlled size and homogeneous shells were first fabricated using capillary microfluidic devices for the purpose of controlling drug release kinetics. Sizes of PLGA cores were readily controlled by the geometries of microfluidic devices and the fluid flow rates. PLGA microspheres with sizes ranging from 15 to 50 μm were fabricated to investigate the influence of the core size on the release kinetics. Rifampicin was loaded into both monodisperse PLGA microspheres and PLGA-alginate core-shell microspheres as a model drug for the release kinetics studies. The in vitro release of rifampicin showed that the PLGA core of all sizes exhibited sigmoid release patterns, although smaller PLGA cores had a higher release rate and a shorter lag phase. The shell could modulate the drug release kinetics as a buffer layer and a near-zero-order release pattern was observed when the drug release rate of the PLGA core was high enough. The biocompatibility of PLGA-alginate core-shell microspheres was assessed by MTT assay on L929 mouse fibroblasts cell line and no obvious cytotoxicity was found. This technique provides a convenient method to control the drug release kinetics of the PLGA microsphere by delicately controlling the microstructures. The obtained monodisperse PLGA-alginate core-shell microspheres with monodisperse size and homogeneous shells could be a promising device for controlled drug release. © 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	7410-7419
Journal	Acta Biomaterialia
Volume	9
Issue number	7
DOIs	https://doi.org/10.1016/j.actbio.2013.03.022
Publication status	Published - Jul 2013
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Funding

We gratefully acknowledge financial support from the Research Grants Council of Hong Kong (GRF718009, GRF718111 and HKU707712P), the Science and Technology Innovation Commission of Shenzhen Municipality (JC201105190878A), National Natural Science Foundation of China (NSFC51206138/E0605) and the Seed Funding from The University of Hong Kong (201109160030 and 201109176165). This research is supported in part by the Zhejiang Provincial, Hangzhou Municipal and Lin’an County Governments.

Research Keywords

Alginate
Capillary microfluidic device
Controlled drug release
Core-shell microspheres
PLGA

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RGC-funded

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10.1016/j.actbio.2013.03.022

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title = "Fabrication and characterization of monodisperse PLGA-alginate core-shell microspheres with monodisperse size and homogeneous shells for controlled drug release",

abstract = "Monodisperse PLGA-alginate core-shell microspheres with controlled size and homogeneous shells were first fabricated using capillary microfluidic devices for the purpose of controlling drug release kinetics. Sizes of PLGA cores were readily controlled by the geometries of microfluidic devices and the fluid flow rates. PLGA microspheres with sizes ranging from 15 to 50 μm were fabricated to investigate the influence of the core size on the release kinetics. Rifampicin was loaded into both monodisperse PLGA microspheres and PLGA-alginate core-shell microspheres as a model drug for the release kinetics studies. The in vitro release of rifampicin showed that the PLGA core of all sizes exhibited sigmoid release patterns, although smaller PLGA cores had a higher release rate and a shorter lag phase. The shell could modulate the drug release kinetics as a buffer layer and a near-zero-order release pattern was observed when the drug release rate of the PLGA core was high enough. The biocompatibility of PLGA-alginate core-shell microspheres was assessed by MTT assay on L929 mouse fibroblasts cell line and no obvious cytotoxicity was found. This technique provides a convenient method to control the drug release kinetics of the PLGA microsphere by delicately controlling the microstructures. The obtained monodisperse PLGA-alginate core-shell microspheres with monodisperse size and homogeneous shells could be a promising device for controlled drug release. {\textcopyright} 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.",

keywords = "Alginate, Capillary microfluidic device, Controlled drug release, Core-shell microspheres, PLGA",

author = "Jun Wu and Tiantian Kong and Yeung, \{Kelvin Wai Kwok\} and Shum, \{Ho Cheung\} and Cheung, \{Kenneth Man Chee\} and Liqiu Wang and To, \{Michael Kai Tsun\}",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].",

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Fabrication and characterization of monodisperse PLGA-alginate core-shell microspheres with monodisperse size and homogeneous shells for controlled drug release. / Wu, Jun; Kong, Tiantian; Yeung, Kelvin Wai Kwok et al.
In: Acta Biomaterialia, Vol. 9, No. 7, 07.2013, p. 7410-7419.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

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T1 - Fabrication and characterization of monodisperse PLGA-alginate core-shell microspheres with monodisperse size and homogeneous shells for controlled drug release

AU - Wu, Jun

AU - Kong, Tiantian

AU - Yeung, Kelvin Wai Kwok

AU - Shum, Ho Cheung

AU - Cheung, Kenneth Man Chee

AU - Wang, Liqiu

AU - To, Michael Kai Tsun

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2013/7

Y1 - 2013/7

N2 - Monodisperse PLGA-alginate core-shell microspheres with controlled size and homogeneous shells were first fabricated using capillary microfluidic devices for the purpose of controlling drug release kinetics. Sizes of PLGA cores were readily controlled by the geometries of microfluidic devices and the fluid flow rates. PLGA microspheres with sizes ranging from 15 to 50 μm were fabricated to investigate the influence of the core size on the release kinetics. Rifampicin was loaded into both monodisperse PLGA microspheres and PLGA-alginate core-shell microspheres as a model drug for the release kinetics studies. The in vitro release of rifampicin showed that the PLGA core of all sizes exhibited sigmoid release patterns, although smaller PLGA cores had a higher release rate and a shorter lag phase. The shell could modulate the drug release kinetics as a buffer layer and a near-zero-order release pattern was observed when the drug release rate of the PLGA core was high enough. The biocompatibility of PLGA-alginate core-shell microspheres was assessed by MTT assay on L929 mouse fibroblasts cell line and no obvious cytotoxicity was found. This technique provides a convenient method to control the drug release kinetics of the PLGA microsphere by delicately controlling the microstructures. The obtained monodisperse PLGA-alginate core-shell microspheres with monodisperse size and homogeneous shells could be a promising device for controlled drug release. © 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

AB - Monodisperse PLGA-alginate core-shell microspheres with controlled size and homogeneous shells were first fabricated using capillary microfluidic devices for the purpose of controlling drug release kinetics. Sizes of PLGA cores were readily controlled by the geometries of microfluidic devices and the fluid flow rates. PLGA microspheres with sizes ranging from 15 to 50 μm were fabricated to investigate the influence of the core size on the release kinetics. Rifampicin was loaded into both monodisperse PLGA microspheres and PLGA-alginate core-shell microspheres as a model drug for the release kinetics studies. The in vitro release of rifampicin showed that the PLGA core of all sizes exhibited sigmoid release patterns, although smaller PLGA cores had a higher release rate and a shorter lag phase. The shell could modulate the drug release kinetics as a buffer layer and a near-zero-order release pattern was observed when the drug release rate of the PLGA core was high enough. The biocompatibility of PLGA-alginate core-shell microspheres was assessed by MTT assay on L929 mouse fibroblasts cell line and no obvious cytotoxicity was found. This technique provides a convenient method to control the drug release kinetics of the PLGA microsphere by delicately controlling the microstructures. The obtained monodisperse PLGA-alginate core-shell microspheres with monodisperse size and homogeneous shells could be a promising device for controlled drug release. © 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

KW - Alginate

KW - Capillary microfluidic device

KW - Controlled drug release

KW - Core-shell microspheres

KW - PLGA

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SN - 1742-7061

VL - 9

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EP - 7419

JO - Acta Biomaterialia

JF - Acta Biomaterialia

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ER -

Fabrication and characterization of monodisperse PLGA-alginate core-shell microspheres with monodisperse size and homogeneous shells for controlled drug release

Abstract

Bibliographical note

Funding

Research Keywords

RGC Funding Information

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