Extended-spectrum β-lactamase-producing E. coli from retail meat and workers: genetic diversity, virulotyping, pathotyping and the antimicrobial effect of silver nanoparticles

Heba A. Ahmed; Ibrahim Elsohaby; Amina M. Elamin; Abeer E Abd El-Ghafar; Gamilat A. Elsaid; Mervat Elbarbary; Rasha A. Mohsen; Tamer M. El Feky; Rasha M. El Bayomi

doi:10.1186/s12866-023-02948-0

Extended-spectrum β-lactamase-producing E. coli from retail meat and workers: genetic diversity, virulotyping, pathotyping and the antimicrobial effect of silver nanoparticles

Heba A. Ahmed^*
, Ibrahim Elsohaby
, Amina M. Elamin
, Abeer E Abd El-Ghafar
, Gamilat A. Elsaid
, Mervat Elbarbary
, Rasha A. Mohsen
, Tamer M. El Feky
, Rasha M. El Bayomi

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

23 Citations (Scopus)

38 Downloads (CityUHK Scholars)

Abstract

Background The spread of extended-spectrum β-lactamases (ESBL) producing E. coli from food animals and the environment to humans has become a significant public health concern. The objectives of this study were to determine the occurrence, pathotypes, virulotypes, genotypes, and antimicrobial resistance patterns of ESBL-producing E. coli in retail meat samples and workers in retail meat shops in Egypt and to evaluate the bactericidal efficacy of silver nanoparticles (AgNPs-H₂O₂) against multidrug resistant (MDR) ESBL-producing E. coli.

Results A total of 250 retail meat samples and 100 human worker samples (hand swabs and stool) were examined for the presence of ESBL- producing E. coli. Duck meat and workers' hand swabs were the highest proportion of ESBL- producing E. coli isolates (81.1%), followed by camel meat (61.5%). Pathotyping revealed that the isolates belonged to groups A and B1. Virulotyping showed that the most prevalent virulence gene was Shiga toxin 2 (stx2) associated gene (36.9%), while none of the isolates harbored stx1 gene. Genotyping of the identified isolates from human and meat sources by REP-PCR showed 100% similarity within the same cluster between human and meat isolates. All isolates were classified as MDR with an average multiple antibiotic resistance (MAR) index of 0.7. AgNPs-H₂O₂ at concentrations of 0.625, 1.25, 2.5 and 5 μg/mL showed complete bacterial growth inhibition.

Conclusions Virulent MDR ESBL-producing E. coli were identified in retail meat products in Egypt, posing significant public health threats. Regular monitoring of ESBL-producing E. coli frequency and antimicrobial resistance profile in retail meat products is crucial to enhance their safety. AgNPs-H₂O₂ is a promising alternative for treating MDR ESBL-producing E. coli infections and reducing antimicrobial resistance risks.

© 2023. BioMed Central Ltd., part of Springer Nature.

Original language	English
Journal	BMC Microbiology
Volume	23
Online published	7 Aug 2023
DOIs	https://doi.org/10.1186/s12866-023-02948-0
Publication status	Published - 2023

Research Keywords

Antimicrobial resistance
E. coli
ESBL
Multidrug resistant
Retail meat
Silver nanoparticles

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Ahmed, H. A., Elsohaby, I., Elamin, A. M., El-Ghafar, A. E. A., Elsaid, G. A., Elbarbary, M., Mohsen, R. A., El Feky, T. M., & El Bayomi, R. M. (2023). Extended-spectrum β-lactamase-producing E. coli from retail meat and workers: genetic diversity, virulotyping, pathotyping and the antimicrobial effect of silver nanoparticles. BMC Microbiology, 23. https://doi.org/10.1186/s12866-023-02948-0

@article{8dad99005dea44df8905b4cf2bf174a8,

title = "Extended-spectrum β-lactamase-producing E. coli from retail meat and workers: genetic diversity, virulotyping, pathotyping and the antimicrobial effect of silver nanoparticles",

abstract = "Background The spread of extended-spectrum β-lactamases (ESBL) producing E. coli from food animals and the environment to humans has become a significant public health concern. The objectives of this study were to determine the occurrence, pathotypes, virulotypes, genotypes, and antimicrobial resistance patterns of ESBL-producing E. coli in retail meat samples and workers in retail meat shops in Egypt and to evaluate the bactericidal efficacy of silver nanoparticles (AgNPs-H2O2) against multidrug resistant (MDR) ESBL-producing E. coli. Results A total of 250 retail meat samples and 100 human worker samples (hand swabs and stool) were examined for the presence of ESBL- producing E. coli. Duck meat and workers' hand swabs were the highest proportion of ESBL- producing E. coli isolates (81.1\%), followed by camel meat (61.5\%). Pathotyping revealed that the isolates belonged to groups A and B1. Virulotyping showed that the most prevalent virulence gene was Shiga toxin 2 (stx2) associated gene (36.9\%), while none of the isolates harbored stx1 gene. Genotyping of the identified isolates from human and meat sources by REP-PCR showed 100\% similarity within the same cluster between human and meat isolates. All isolates were classified as MDR with an average multiple antibiotic resistance (MAR) index of 0.7. AgNPs-H2O2 at concentrations of 0.625, 1.25, 2.5 and 5 μg/mL showed complete bacterial growth inhibition. Conclusions Virulent MDR ESBL-producing E. coli were identified in retail meat products in Egypt, posing significant public health threats. Regular monitoring of ESBL-producing E. coli frequency and antimicrobial resistance profile in retail meat products is crucial to enhance their safety. AgNPs-H2O2 is a promising alternative for treating MDR ESBL-producing E. coli infections and reducing antimicrobial resistance risks. {\textcopyright} 2023. BioMed Central Ltd., part of Springer Nature.",

keywords = "Antimicrobial resistance, E. coli, ESBL, Multidrug resistant, Retail meat, Silver nanoparticles",

author = "Ahmed, \{Heba A.\} and Ibrahim Elsohaby and Elamin, \{Amina M.\} and El-Ghafar, \{Abeer E Abd\} and Elsaid, \{Gamilat A.\} and Mervat Elbarbary and Mohsen, \{Rasha A.\} and \{El Feky\}, \{Tamer M.\} and \{El Bayomi\}, \{Rasha M.\}",

year = "2023",

doi = "10.1186/s12866-023-02948-0",

language = "English",

volume = "23",

journal = "BMC Microbiology",

issn = "1471-2180",

publisher = "BioMed Central Ltd.",

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Ahmed, HA, Elsohaby, I, Elamin, AM, El-Ghafar, AEA, Elsaid, GA, Elbarbary, M, Mohsen, RA, El Feky, TM & El Bayomi, RM 2023, 'Extended-spectrum β-lactamase-producing E. coli from retail meat and workers: genetic diversity, virulotyping, pathotyping and the antimicrobial effect of silver nanoparticles', BMC Microbiology, vol. 23. https://doi.org/10.1186/s12866-023-02948-0

Extended-spectrum β-lactamase-producing E. coli from retail meat and workers: genetic diversity, virulotyping, pathotyping and the antimicrobial effect of silver nanoparticles. / Ahmed, Heba A.; Elsohaby, Ibrahim; Elamin, Amina M. et al.
In: BMC Microbiology, Vol. 23, 2023.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

TY - JOUR

T1 - Extended-spectrum β-lactamase-producing E. coli from retail meat and workers

T2 - genetic diversity, virulotyping, pathotyping and the antimicrobial effect of silver nanoparticles

AU - Ahmed, Heba A.

AU - Elsohaby, Ibrahim

AU - Elamin, Amina M.

AU - El-Ghafar, Abeer E Abd

AU - Elsaid, Gamilat A.

AU - Elbarbary, Mervat

AU - Mohsen, Rasha A.

AU - El Feky, Tamer M.

AU - El Bayomi, Rasha M.

PY - 2023

Y1 - 2023

N2 - Background The spread of extended-spectrum β-lactamases (ESBL) producing E. coli from food animals and the environment to humans has become a significant public health concern. The objectives of this study were to determine the occurrence, pathotypes, virulotypes, genotypes, and antimicrobial resistance patterns of ESBL-producing E. coli in retail meat samples and workers in retail meat shops in Egypt and to evaluate the bactericidal efficacy of silver nanoparticles (AgNPs-H2O2) against multidrug resistant (MDR) ESBL-producing E. coli. Results A total of 250 retail meat samples and 100 human worker samples (hand swabs and stool) were examined for the presence of ESBL- producing E. coli. Duck meat and workers' hand swabs were the highest proportion of ESBL- producing E. coli isolates (81.1%), followed by camel meat (61.5%). Pathotyping revealed that the isolates belonged to groups A and B1. Virulotyping showed that the most prevalent virulence gene was Shiga toxin 2 (stx2) associated gene (36.9%), while none of the isolates harbored stx1 gene. Genotyping of the identified isolates from human and meat sources by REP-PCR showed 100% similarity within the same cluster between human and meat isolates. All isolates were classified as MDR with an average multiple antibiotic resistance (MAR) index of 0.7. AgNPs-H2O2 at concentrations of 0.625, 1.25, 2.5 and 5 μg/mL showed complete bacterial growth inhibition. Conclusions Virulent MDR ESBL-producing E. coli were identified in retail meat products in Egypt, posing significant public health threats. Regular monitoring of ESBL-producing E. coli frequency and antimicrobial resistance profile in retail meat products is crucial to enhance their safety. AgNPs-H2O2 is a promising alternative for treating MDR ESBL-producing E. coli infections and reducing antimicrobial resistance risks. © 2023. BioMed Central Ltd., part of Springer Nature.

AB - Background The spread of extended-spectrum β-lactamases (ESBL) producing E. coli from food animals and the environment to humans has become a significant public health concern. The objectives of this study were to determine the occurrence, pathotypes, virulotypes, genotypes, and antimicrobial resistance patterns of ESBL-producing E. coli in retail meat samples and workers in retail meat shops in Egypt and to evaluate the bactericidal efficacy of silver nanoparticles (AgNPs-H2O2) against multidrug resistant (MDR) ESBL-producing E. coli. Results A total of 250 retail meat samples and 100 human worker samples (hand swabs and stool) were examined for the presence of ESBL- producing E. coli. Duck meat and workers' hand swabs were the highest proportion of ESBL- producing E. coli isolates (81.1%), followed by camel meat (61.5%). Pathotyping revealed that the isolates belonged to groups A and B1. Virulotyping showed that the most prevalent virulence gene was Shiga toxin 2 (stx2) associated gene (36.9%), while none of the isolates harbored stx1 gene. Genotyping of the identified isolates from human and meat sources by REP-PCR showed 100% similarity within the same cluster between human and meat isolates. All isolates were classified as MDR with an average multiple antibiotic resistance (MAR) index of 0.7. AgNPs-H2O2 at concentrations of 0.625, 1.25, 2.5 and 5 μg/mL showed complete bacterial growth inhibition. Conclusions Virulent MDR ESBL-producing E. coli were identified in retail meat products in Egypt, posing significant public health threats. Regular monitoring of ESBL-producing E. coli frequency and antimicrobial resistance profile in retail meat products is crucial to enhance their safety. AgNPs-H2O2 is a promising alternative for treating MDR ESBL-producing E. coli infections and reducing antimicrobial resistance risks. © 2023. BioMed Central Ltd., part of Springer Nature.

KW - Antimicrobial resistance

KW - E. coli

KW - ESBL

KW - Multidrug resistant

KW - Retail meat

KW - Silver nanoparticles

UR - https://www.scopus.com/pages/publications/85166784395

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85166784395&origin=recordpage

U2 - 10.1186/s12866-023-02948-0

DO - 10.1186/s12866-023-02948-0

M3 - RGC 21 - Publication in refereed journal

C2 - 37550643

SN - 1471-2180

VL - 23

JO - BMC Microbiology

JF - BMC Microbiology

ER -

Extended-spectrum β-lactamase-producing E. coli from retail meat and workers: genetic diversity, virulotyping, pathotyping and the antimicrobial effect of silver nanoparticles

Abstract

Research Keywords

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