TY - JOUR
T1 - Exploring bacterial communities through metagenomics during bioremediation of polycyclic aromatic hydrocarbons from contaminated sediments
AU - Gosai, Haren B.
AU - Panseriya, Haresh Z.
AU - Patel, Payal G.
AU - Patel, Ajay C.
AU - Shankar, Alka
AU - Varjani, Sunita
AU - Dave, Bharti P.
PY - 2022/10/10
Y1 - 2022/10/10
N2 - The goal of this study was to evaluate the degradation effectiveness of PAHs degrading bacteria at the mesocosm level, including Stenotrophomonas maltophilia (SC), mixed culture (MC), and enriched native microflora (EC) at the mesocosm level. Maximum degradation was found in the mesocosm MC (26.67 %), followed by SC (25.08 %) and EC (18.25 %) after 60 days. Thus, mixed culture and Stenotrophomonas maltophilia could be a game changer in the PAHs bioremediation at the chronically contaminated sites. MiSeq sequencing has revealed dominancy of γ-Proteobacteria, α-Proteobacteria, β-Proteobacteria at class level and Sphingomonadales, oceanospirillales, Rhodothermales at Order level. Families Alcanivoracaceae, Alteromonadaceae, Nocardiaceae, Rhodospirillaceae and genus Stenotrophomonas, Alcanivorax, Methylophaga, Fluviicola and Rhodoplanes were considerably increased which play key role in the PAHs degradation. Dominant bacterial communities have revealed resilience community to enable potential PAHs degradation process in all the mesocosms. To the best our knowledge this is the first ever attempt in PAHs biodegradation study conducted at the mesocosm level mimicking natural environmental conditions. Consequently, this study could be a benchmark against which future progress studies for the policy makers and stakeholders to design appropriate bioremediation study for the historically PAHs polluted contaminate sites.© 2022 Elsevier B.V. All rights reserved.
AB - The goal of this study was to evaluate the degradation effectiveness of PAHs degrading bacteria at the mesocosm level, including Stenotrophomonas maltophilia (SC), mixed culture (MC), and enriched native microflora (EC) at the mesocosm level. Maximum degradation was found in the mesocosm MC (26.67 %), followed by SC (25.08 %) and EC (18.25 %) after 60 days. Thus, mixed culture and Stenotrophomonas maltophilia could be a game changer in the PAHs bioremediation at the chronically contaminated sites. MiSeq sequencing has revealed dominancy of γ-Proteobacteria, α-Proteobacteria, β-Proteobacteria at class level and Sphingomonadales, oceanospirillales, Rhodothermales at Order level. Families Alcanivoracaceae, Alteromonadaceae, Nocardiaceae, Rhodospirillaceae and genus Stenotrophomonas, Alcanivorax, Methylophaga, Fluviicola and Rhodoplanes were considerably increased which play key role in the PAHs degradation. Dominant bacterial communities have revealed resilience community to enable potential PAHs degradation process in all the mesocosms. To the best our knowledge this is the first ever attempt in PAHs biodegradation study conducted at the mesocosm level mimicking natural environmental conditions. Consequently, this study could be a benchmark against which future progress studies for the policy makers and stakeholders to design appropriate bioremediation study for the historically PAHs polluted contaminate sites.© 2022 Elsevier B.V. All rights reserved.
KW - Gulf of Kutch
KW - Mesocosm
KW - MiSeq
KW - Mixed culture
KW - Polycyclic aromatic hydrocarbons
UR - http://www.scopus.com/inward/record.url?scp=85133720782&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85133720782&origin=recordpage
U2 - 10.1016/j.scitotenv.2022.156794
DO - 10.1016/j.scitotenv.2022.156794
M3 - RGC 21 - Publication in refereed journal
C2 - 35738384
SN - 0048-9697
VL - 842
JO - Science of the Total Environment
JF - Science of the Total Environment
M1 - 156794
ER -
