Exploitation of Environment-sensitive Luminophores in the Design of Sydnone-based Bioorthogonal Imaging Reagents

Research output: Conference Papers (RGC: 31A, 31B, 32, 33)33_Other conference paper

View graph of relations

Author(s)

Related Research Unit(s)

Detail(s)

Original languageEnglish
Publication statusPresented - 4 May 2019

Conference

Title26th Symposium on Chemistry Postgraduate Research in Hong Kong
LocationChinese University of Hong Kong
PlaceHong Kong
Period4 May 2019

Abstract

Bioorthogonal probes have been developed as versatile tools for the specific labeling and imaging of biomolecules in their native environments. Recently, sydnone derivatives have been identified as new bioorthogonal reaction partners of alkynes in [3+2] cycloaddition reactions. They undergo strain-promoted sydnone–alkyne cycloaddition (SPSAC) reaction with strained alkynes, resulting in the formation of substituted pyrazoles as the products with the extrusion of carbon dioxide. With our ongoing interest in the development of luminescent transition metal complexes as cellular probes and bioimaging reagents, we modified sydnone with cyclometalated iridium(III) polypyridine complexes to afford novel bioorthogonal imaging reagents with highly environment-sensitive emission properties. These complexes displayed significant emission enhancement upon SPSAC reaction in aqueous solution, and were utilized to label alkyne-modified proteins and image ceramide molecules in live cells.  

Citation Format(s)

Exploitation of Environment-sensitive Luminophores in the Design of Sydnone-based Bioorthogonal Imaging Reagents. / Lee, Lawrence Cho-Cheung ; Cheung, Hugo Man-Hin; Liu, Hua-Wei; Lo, Kenneth Kam-Wing.

2019. 26th Symposium on Chemistry Postgraduate Research in Hong Kong, Hong Kong.

Research output: Conference Papers (RGC: 31A, 31B, 32, 33)33_Other conference paper