Experimental setup for studying the effects of alpha particles on zebrafish embryos

E. H W Yum; C. K M Ng; A. C C Lin; S. H. Cheng; K. N. Yu

doi:10.1016/j.nimb.2007.07.024

Author(s)

E. H W Yum
C. K M Ng
A. C C Lin
S. H. Cheng
K. N. Yu

Detail(s)

Original language	English
Pages (from-to)	171-176
Journal / Publication	Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
Volume	264
Issue number	1
Publication status	Published - Nov 2007

Link(s)

DOI	DOI https://doi.org/10.1016/j.nimb.2007.07.024 Final Published version
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Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-35448954870&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(f78f99d6-b5cd-4ebd-8cf3-06ab8039a9bc).html

Abstract

In the present work, we have studied the feasibility to use an experimental setup based on polyallyldiglycol-carbonate (PADC) films to study effects of alpha particles on dechorionated zebrafish embryos. Thin PADC films with a thickness of 16 μm were prepared from commercially available CR-39 films by chemical etching and used as support substrates for holding zebrafish embryos for alpha-particle irradiation. These films recorded alpha-particle hit positions, quantified the number and energy of alpha particles actually incident on the embryo cells, and thus enabled the calculation of the dose absorbed by the embryo cells. Irradiation was made at 4 h post fertilization (hpf) with absorbed doses up to 2.3 mGy. Images of the embryos at 48 hpf were examined for identification of morphologic abnormalities. The preliminary results showed that absorbed doses corresponding to the abnormally developed embryos ranged from 0.41 to 2.3 mGy, which was equivalent to 0.21-1.2 mGy in human. © 2007 Elsevier B.V. All rights reserved.

Research Area(s)

Alpha particle, CR-39, Danio rerio, PADC, Solid-state nuclear track detector, SSNTD, Zebrafish embryos

Citation Format(s)

[ RIS ] [ BibTeX ]

Experimental setup for studying the effects of alpha particles on zebrafish embryos. / Yum, E. H W; Ng, C. K M; Lin, A. C C et al.
In: Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, Vol. 264, No. 1, 11.2007, p. 171-176.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Yum, EHW, Ng, CKM, Lin, ACC, Cheng, SH & Yu, KN 2007, 'Experimental setup for studying the effects of alpha particles on zebrafish embryos', Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, vol. 264, no. 1, pp. 171-176. https://doi.org/10.1016/j.nimb.2007.07.024

Yum, E. H. W., Ng, C. K. M., Lin, A. C. C., Cheng, S. H., & Yu, K. N. (2007). Experimental setup for studying the effects of alpha particles on zebrafish embryos. Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, 264(1), 171-176. https://doi.org/10.1016/j.nimb.2007.07.024

Yum EHW, Ng CKM, Lin ACC, Cheng SH , Yu KN. Experimental setup for studying the effects of alpha particles on zebrafish embryos. Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms. 2007 Nov;264(1):171-176. doi: 10.1016/j.nimb.2007.07.024

Yum, E. H W ; Ng, C. K M ; Lin, A. C C et al. / Experimental setup for studying the effects of alpha particles on zebrafish embryos. In: Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms. 2007 ; Vol. 264, No. 1. pp. 171-176.