Experiences with infectious cDNA clones of equine arteritis virus: Lessons learned and insights gained

Udeni B.R. Balasuriya; Jianqiang Zhang; Yun Young Go; N. James MacLachlan

doi:10.1016/j.virol.2014.04.029

Experiences with infectious cDNA clones of equine arteritis virus : Lessons learned and insights gained

Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review

Overview

5 Scopus Citations

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Author(s)

Udeni B.R. Balasuriya
Jianqiang Zhang
Yun Young Go
N. James MacLachlan

Detail(s)

Original language	English
Pages (from-to)	388-403
Journal / Publication	Virology
Volume	462-463
Online published	7 Jun 2014
Publication status	Published - Aug 2014
Externally published	Yes

Link(s)

DOI	DOI https://doi.org/10.1016/j.virol.2014.04.029 Final Published version
	Check@CityULib
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-84905385358&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(c57c9f74-d06e-4bd5-be47-4154cb674c96).html

Abstract

The advent of recombinant DNA technology, development of infectious cDNA clones of RNA viruses, and reverse genetic technologies have revolutionized how viruses are studied. Genetic manipulation of full-length cDNA clones has become an especially important and widely used tool to study the biology, pathogenesis, and virulence determinants of both positive and negative stranded RNA viruses. The first full-length infectious cDNA clone of equine arteritis virus (EAV) was developed in 1996 and was also the first full-length infectious cDNA clone constructed from a member of the order Nidovirales. This clone was extensively used to characterize the molecular biology of EAV and other Nidoviruses. The objective of this review is to summarize the characterization of the virulence (or attenuation) phenotype of the recombinant viruses derived from several infectious cDNA clones of EAV in horses, as well as their application for characterization of the molecular basis of viral neutralization, persistence, and cellular tropism.

Research Area(s)

Arteriviruses, CDNA clones, EAV, Equine arteritis virus, Equine viral arteritis, EVA, Reverse genetics

Citation Format(s)

[ RIS ] [ BibTeX ]

Experiences with infectious cDNA clones of equine arteritis virus: Lessons learned and insights gained. / Balasuriya, Udeni B.R.; Zhang, Jianqiang; Go, Yun Young et al.
In: Virology, Vol. 462-463, 08.2014, p. 388-403.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review

Balasuriya, UBR, Zhang, J, Go, YY & MacLachlan, NJ 2014, 'Experiences with infectious cDNA clones of equine arteritis virus: Lessons learned and insights gained', Virology, vol. 462-463, pp. 388-403. https://doi.org/10.1016/j.virol.2014.04.029

Balasuriya, U. B. R., Zhang, J., Go, Y. Y., & MacLachlan, N. J. (2014). Experiences with infectious cDNA clones of equine arteritis virus: Lessons learned and insights gained. Virology, 462-463, 388-403. https://doi.org/10.1016/j.virol.2014.04.029

Balasuriya UBR, Zhang J, Go YY, MacLachlan NJ. Experiences with infectious cDNA clones of equine arteritis virus: Lessons learned and insights gained. Virology. 2014 Aug;462-463:388-403. Epub 2014 Jun 7. doi: 10.1016/j.virol.2014.04.029

Balasuriya, Udeni B.R. ; Zhang, Jianqiang ; Go, Yun Young et al. / Experiences with infectious cDNA clones of equine arteritis virus : Lessons learned and insights gained. In: Virology. 2014 ; Vol. 462-463. pp. 388-403.