Evolution and comparative genomics of pAQU-like conjugative plasmids in Vibrio species

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

6 Scopus Citations
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Author(s)

  • Ruichao Li
  • Lianwei Ye
  • Marcus Ho Yin Wong
  • Zhiwei Zheng
  • Edward Wai Chi Chan

Detail(s)

Original languageEnglish
Pages (from-to)2503-2506
Journal / PublicationJournal of Antimicrobial Chemotherapy
Volume72
Issue number9
Online published20 Jun 2017
Publication statusPublished - Sep 2017
Externally publishedYes

Abstract

Objectives: To investigate a set of MDR conjugative plasmids found in Vibrio species and characterize the underlying evolution process. Methods: pAQU-type plasmids from Vibrio species were sequenced using both Illumina and PacBio platforms. Bioinformatics tools were utilized to analyse the typical MDR regions and core genes in the plasmids. Results: The nine pAQU-type plasmids ranged from ~160 to 206 kb in size and were found to harbour as many as 111 core genes encoding conjugative, replication and maintenance functions. Eight plasmids were found to carry a typical MDR region, which contained various accessory and resistance genes, including ISCR1-blaPER-1-bearing complex class 1 integrons, ISCR2-floR, ISCR2-tet(D)-tetR-ISCR2, qnrVC6, a Tn10-like structure and others associated with mobile elements. Comparison between a plasmid without resistance genes and different MDR plasmids showed that integration of different mobile elements, such as IS26, ISCR1, ISCR2, IS10 and IS6100, into the plasmid backbone was the key mechanism by which foreign resistance genes were acquired during the evolution process. Conclusions: This study identified pAQU-type plasmids as emerging MDR conjugative plasmids among important pathogens from different origins in Asia. These findings suggest that aquatic bacteria constitute a major reservoir of resistance genes, which may be transmissible to other human pathogens during food production and processing.

Citation Format(s)

Evolution and comparative genomics of pAQU-like conjugative plasmids in Vibrio species. / Li, Ruichao; Ye, Lianwei; Wong, Marcus Ho Yin; Zheng, Zhiwei; Chan, Edward Wai Chi; Chen, Sheng.

In: Journal of Antimicrobial Chemotherapy, Vol. 72, No. 9, 09.2017, p. 2503-2506.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review