Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detection in Semen
Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review
Author(s)
Detail(s)
| Original language | English |
|---|---|
| Pages (from-to) | 3694-3696 |
| Journal / Publication | Journal of Clinical Microbiology |
| Volume | 49 |
| Issue number | 10 |
| Online published | 29 Sep 2011 |
| Publication status | Published - Oct 2011 |
| Externally published | Yes |
Link(s)
Abstract
This study showed that under specifically defined conditions with respect to nucleic acid extraction method and testing reagents, a previously described real-time reverse transcription-PCR (rRT-PCR) assay (T1 assay) provides sensitivity equal to or higher than that of virus isolation for the detection of equine arteritis virus in semen.
Citation Format(s)
Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detection in Semen. / Miszczak, Fabien; Shuck, Kathleen M.; Lu, Zhengchun; Go, Yun Young; Zhang, Jianqiang; Sells, Stephen; Vabret, Astrid; Pronost, Stéphane; Fortier, Guillaume; Timoney, Peter J.; Balasuriya, Udeni B. R.
In: Journal of Clinical Microbiology, Vol. 49, No. 10, 10.2011, p. 3694-3696.Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review
