Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detection in Semen

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Fabien Miszczak
  • Kathleen M. Shuck
  • Zhengchun Lu
  • Jianqiang Zhang
  • Stephen Sells
  • Astrid Vabret
  • Stéphane Pronost
  • Guillaume Fortier
  • Peter J. Timoney
  • Udeni B. R. Balasuriya

Detail(s)

Original languageEnglish
Pages (from-to)3694-3696
Journal / PublicationJournal of Clinical Microbiology
Volume49
Issue number10
Online published29 Sep 2011
Publication statusPublished - Oct 2011
Externally publishedYes

Abstract

This study showed that under specifically defined conditions with respect to nucleic acid extraction method and testing reagents, a previously described real-time reverse transcription-PCR (rRT-PCR) assay (T1 assay) provides sensitivity equal to or higher than that of virus isolation for the detection of equine arteritis virus in semen.

Citation Format(s)

Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detection in Semen. / Miszczak, Fabien; Shuck, Kathleen M.; Lu, Zhengchun; Go, Yun Young; Zhang, Jianqiang; Sells, Stephen; Vabret, Astrid; Pronost, Stéphane; Fortier, Guillaume; Timoney, Peter J.; Balasuriya, Udeni B. R.

In: Journal of Clinical Microbiology, Vol. 49, No. 10, 10.2011, p. 3694-3696.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review