TY - JOUR
T1 - Evaluation of porous bacterial cellulose produced from foam templating with different additives and its application in 3D cell culture
AU - Lin, Shin-Ping
AU - Singajaya, Stephanie
AU - Lo, Tsui-Yun
AU - Santoso, Shella Permatasari
AU - Hsu, Hsien-Yi
AU - Cheng, Kuan-Chen
PY - 2023/4/15
Y1 - 2023/4/15
N2 - Bacterial cellulose (BC) is used in biomedical applications due to its unique material properties such as mechanical strength with a high water-absorbing capacity and biocompatibility. Nevertheless, native BC lacks porosity control which is crucial for regenerative medicine. Hence, developing a simple technique to change the pore sizes of BC has become an important issue. This study combined current foaming BC (FBC) production with incorporation of different additives (avicel, carboxymethylcellulose, and chitosan) to form novel porous additive-altered FBC. Results demonstrated that the FBC samples provided greater reswelling rates (91.57 % ~ 93.67 %) compared to BC samples (44.52 % ~ 67.5 %). Moreover, the FBC samples also showed excellent cell adhesion and proliferation abilities for NIH-3T3 cells. Lastly, FBC allowed cells to penetrate to deep layers for cell adhesion due to its porous structure, providing a competitive scaffold for 3D cell culture in tissue engineering. © 2023 Elsevier B.V. All rights reserved.
AB - Bacterial cellulose (BC) is used in biomedical applications due to its unique material properties such as mechanical strength with a high water-absorbing capacity and biocompatibility. Nevertheless, native BC lacks porosity control which is crucial for regenerative medicine. Hence, developing a simple technique to change the pore sizes of BC has become an important issue. This study combined current foaming BC (FBC) production with incorporation of different additives (avicel, carboxymethylcellulose, and chitosan) to form novel porous additive-altered FBC. Results demonstrated that the FBC samples provided greater reswelling rates (91.57 % ~ 93.67 %) compared to BC samples (44.52 % ~ 67.5 %). Moreover, the FBC samples also showed excellent cell adhesion and proliferation abilities for NIH-3T3 cells. Lastly, FBC allowed cells to penetrate to deep layers for cell adhesion due to its porous structure, providing a competitive scaffold for 3D cell culture in tissue engineering. © 2023 Elsevier B.V. All rights reserved.
KW - 3D cell culture
KW - Bacterial cellulose
KW - Foam templating
KW - Tissue scaffold
UR - http://www.scopus.com/inward/record.url?scp=85148343020&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85148343020&origin=recordpage
U2 - 10.1016/j.ijbiomac.2023.123680
DO - 10.1016/j.ijbiomac.2023.123680
M3 - RGC 21 - Publication in refereed journal
SN - 0141-8130
VL - 234
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
M1 - 123680
ER -