Evaluation of a SARS-CoV-2 Surrogate Virus Neutralization Test for Detection of Antibody in Human, Canine, Cat, and Hamster Sera

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Ranawaka A. P. M. Perera
  • Ronald Ko
  • Owen T. Y. Tsang
  • David S. C. Hui
  • Mike Y. M. Kwan
  • Christopher J. Brackman
  • Esther M. W. To
  • Hui-ling Yen
  • Kathy Leung
  • Samuel M. S. Cheng
  • Kin Ho Chan
  • Karl C. K. Chan
  • Ka-Chi Li
  • Linda Saif
  • Joseph T. Wu
  • Thomas H. C. Sit
  • Leo L. M. Poon
  • Malik Peirisa

Detail(s)

Original languageEnglish
Article numbere02504-20
Journal / PublicationJournal of Clinical Microbiology
Volume59
Issue number2
Online published21 Jan 2021
Publication statusPublished - Feb 2021

Abstract

Surrogate neutralization assays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that can be done without biosafety level 3 containment and in multiple species are desirable. We evaluate a recently developed surrogate virus neutralization test (sVNT) in comparison to 90% plaque reduction neutralization tests (PRNT90) in human, canine, cat, and hamster sera. With PRNT90 as the reference, sVNT had sensitivity of 98.9% and specificity of 98.8%. Using a panel of immune sera corresponding to other coronaviruses, we confirm the lack of cross-reactivity to other coronaviruses in SARS-CoV-2 sVNT and PRNT90, except for cross-reactivity to SARS-CoV-1 in sVNT.

Research Area(s)

  • Animal, Antibody, Canine, Cat, COVID-19, Hamster, Human, Neutralization, SARS coronavirus 2, SARS-CoV-2, Seroepidemiology, Serology, Surrogate virus neutralization

Citation Format(s)

Evaluation of a SARS-CoV-2 Surrogate Virus Neutralization Test for Detection of Antibody in Human, Canine, Cat, and Hamster Sera. / Perera, Ranawaka A. P. M.; Ko, Ronald; Tsang, Owen T. Y.; Hui, David S. C.; Kwan, Mike Y. M.; Brackman, Christopher J.; To, Esther M. W.; Yen, Hui-ling; Leung, Kathy; Cheng, Samuel M. S.; Chan, Kin Ho; Chan, Karl C. K.; Li, Ka-Chi; Saif, Linda; Barrs, Vanessa R.; Wu, Joseph T.; Sit, Thomas H. C.; Poon, Leo L. M.; Peirisa, Malik.

In: Journal of Clinical Microbiology, Vol. 59, No. 2, e02504-20, 02.2021.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review