Establishment of a real-time Recombinase Polymerase Amplification (RPA) for the detection of decapod iridescent virus 1 (DIV1)

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Yu Chen
  • Hong Liu
  • Shuai Gao
  • Brett MacKinnon
  • Song-qi Zhu
  • Zhi-qing Wen
  • Peng Jia
  • Xiao-cong Zheng

Detail(s)

Original languageEnglish
Pages (from-to)114377
Journal / PublicationJournal of Virological Methods
Volume300
Online published23 Nov 2021
Publication statusPublished - Feb 2022

Abstract

A rapid and simple real-time recombinase polymerase amplification (RPA) assay was developed to detect decapod iridescent virus 1 (DIV1). The assay was developed using optimized primers and probes designed from the conserved sequence of the DIV1 major capsid protein (MCP) gene. Using the optimized RPA assay, the DIV1 test was completed within 20 min at 39 ℃. The RPA assay was specific to DIV1 with a detection limit of 2.3 × 101 copies/reaction and there was no cross-reactivity with the other aquatic pathogens (WSSV, IHHNV, NHPB, VpAHPND, EHP, IMNV, YHV-1 and GAV) tested. Four out of 45 field-collected shrimp samples tested positive for DIV1 by real-time RPA. The same assay results were obtained by both methods. Thus, the real-time RPA assay developed could be a simple, rapid, sensitive, reliable and affordable method for the on-site diagnosis of DIV1 infection and has significant potential in helping to control DIV1 infections and reduce economic losses to the shrimp industry.

Research Area(s)

  • Recombinase polymerase amplification, On-site detection, Decapod iridescent virus 1 (DIV1)

Citation Format(s)

Establishment of a real-time Recombinase Polymerase Amplification (RPA) for the detection of decapod iridescent virus 1 (DIV1). / Huang, Qian-jun; Chen, Yu; Liu, Hong; St-Hilaire, Sophie; Gao, Shuai; MacKinnon, Brett; Zhu, Song-qi; Wen, Zhi-qing; Jia, Peng; Zheng, Xiao-cong.

In: Journal of Virological Methods, Vol. 300, 02.2022, p. 114377.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review