Epidemiological and phylogenetic analysis reveals Flavobacteriaceae as potential ancestral source of tigecycline resistance gene tet(X)

Rong Zhang; Ning Dong; Zhangqi Shen; Yu Zeng; Jiauyue Lu; Congcong Liu; Hongwei Zhou; Yanyan Hu; Qiaoling Sun; Qipeng Cheng; Lingbing Shu; Jiachang Cai; Edward Wai-Chi Chan; Gongxiang Chen; Sheng Chen

doi:10.1038/s41467-020-18475-9

Epidemiological and phylogenetic analysis reveals Flavobacteriaceae as potential ancestral source of tigecycline resistance gene tet(X)

Rong Zhang, Ning Dong, Zhangqi Shen, Yu Zeng, Jiauyue Lu, Congcong Liu, Hongwei Zhou, Yanyan Hu, Qiaoling Sun, Qipeng Cheng, Lingbing Shu, Jiachang Cai, Edward Wai-Chi Chan, Gongxiang Chen^*, Sheng Chen^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

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Abstract

Emergence of tigecycline-resistance tet(X) gene orthologues rendered tigecycline ineffective as last-resort antibiotic. To understand the potential origin and transmission mechanisms of these genes, we survey the prevalence of tet(X) and its orthologues in 2997 clinical E. coli and K. pneumoniae isolates collected nationwide in China with results showing very low prevalence on these two types of strains, 0.32% and 0%, respectively. Further surveillance of tet(X) orthologues in 3692 different clinical Gram-negative bacterial strains collected during 1994-2019 in hospitals in Zhejiang province, China reveals 106 (2.7%) tet(X)-bearing strains with Flavobacteriaceae being the dominant (97/376, 25.8%) bacteria. In addition, tet(X)s are found to be predominantly located on the chromosomes of Flavobacteriaceae and share similar GC-content as Flavobacteriaceae. It also further evolves into different orthologues and transmits among different species. Data from this work suggest that Flavobacteriaceae could be the potential ancestral source of the tigecycline resistance gene tet(X).

Original language	English
Article number	4648
Journal	Nature Communications
Volume	11
Online published	16 Sept 2020
DOIs	https://doi.org/10.1038/s41467-020-18475-9
Publication status	Published - 2020

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This full text is made available under CC-BY 4.0. https://creativecommons.org/licenses/by/4.0/

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Zhang, R., Dong, N., Shen, Z., Zeng, Y., Lu, J., Liu, C., Zhou, H., Hu, Y., Sun, Q., Cheng, Q., Shu, L., Cai, J., Chan, E. W.-C., Chen, G., & Chen, S. (2020). Epidemiological and phylogenetic analysis reveals Flavobacteriaceae as potential ancestral source of tigecycline resistance gene tet(X). Nature Communications, 11, Article 4648. https://doi.org/10.1038/s41467-020-18475-9

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title = "Epidemiological and phylogenetic analysis reveals Flavobacteriaceae as potential ancestral source of tigecycline resistance gene tet(X)",

abstract = "Emergence of tigecycline-resistance tet(X) gene orthologues rendered tigecycline ineffective as last-resort antibiotic. To understand the potential origin and transmission mechanisms of these genes, we survey the prevalence of tet(X) and its orthologues in 2997 clinical E. coli and K. pneumoniae isolates collected nationwide in China with results showing very low prevalence on these two types of strains, 0.32% and 0%, respectively. Further surveillance of tet(X) orthologues in 3692 different clinical Gram-negative bacterial strains collected during 1994-2019 in hospitals in Zhejiang province, China reveals 106 (2.7%) tet(X)-bearing strains with Flavobacteriaceae being the dominant (97/376, 25.8%) bacteria. In addition, tet(X)s are found to be predominantly located on the chromosomes of Flavobacteriaceae and share similar GC-content as Flavobacteriaceae. It also further evolves into different orthologues and transmits among different species. Data from this work suggest that Flavobacteriaceae could be the potential ancestral source of the tigecycline resistance gene tet(X).",

author = "Rong Zhang and Ning Dong and Zhangqi Shen and Yu Zeng and Jiauyue Lu and Congcong Liu and Hongwei Zhou and Yanyan Hu and Qiaoling Sun and Qipeng Cheng and Lingbing Shu and Jiachang Cai and Chan, {Edward Wai-Chi} and Gongxiang Chen and Sheng Chen",

year = "2020",

doi = "10.1038/s41467-020-18475-9",

language = "English",

volume = "11",

journal = "Nature Communications",

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T1 - Epidemiological and phylogenetic analysis reveals Flavobacteriaceae as potential ancestral source of tigecycline resistance gene tet(X)

AU - Zhang, Rong

AU - Dong, Ning

AU - Shen, Zhangqi

AU - Zeng, Yu

AU - Lu, Jiauyue

AU - Liu, Congcong

AU - Zhou, Hongwei

AU - Hu, Yanyan

AU - Sun, Qiaoling

AU - Cheng, Qipeng

AU - Shu, Lingbing

AU - Cai, Jiachang

AU - Chan, Edward Wai-Chi

AU - Chen, Gongxiang

AU - Chen, Sheng

PY - 2020

Y1 - 2020

N2 - Emergence of tigecycline-resistance tet(X) gene orthologues rendered tigecycline ineffective as last-resort antibiotic. To understand the potential origin and transmission mechanisms of these genes, we survey the prevalence of tet(X) and its orthologues in 2997 clinical E. coli and K. pneumoniae isolates collected nationwide in China with results showing very low prevalence on these two types of strains, 0.32% and 0%, respectively. Further surveillance of tet(X) orthologues in 3692 different clinical Gram-negative bacterial strains collected during 1994-2019 in hospitals in Zhejiang province, China reveals 106 (2.7%) tet(X)-bearing strains with Flavobacteriaceae being the dominant (97/376, 25.8%) bacteria. In addition, tet(X)s are found to be predominantly located on the chromosomes of Flavobacteriaceae and share similar GC-content as Flavobacteriaceae. It also further evolves into different orthologues and transmits among different species. Data from this work suggest that Flavobacteriaceae could be the potential ancestral source of the tigecycline resistance gene tet(X).

AB - Emergence of tigecycline-resistance tet(X) gene orthologues rendered tigecycline ineffective as last-resort antibiotic. To understand the potential origin and transmission mechanisms of these genes, we survey the prevalence of tet(X) and its orthologues in 2997 clinical E. coli and K. pneumoniae isolates collected nationwide in China with results showing very low prevalence on these two types of strains, 0.32% and 0%, respectively. Further surveillance of tet(X) orthologues in 3692 different clinical Gram-negative bacterial strains collected during 1994-2019 in hospitals in Zhejiang province, China reveals 106 (2.7%) tet(X)-bearing strains with Flavobacteriaceae being the dominant (97/376, 25.8%) bacteria. In addition, tet(X)s are found to be predominantly located on the chromosomes of Flavobacteriaceae and share similar GC-content as Flavobacteriaceae. It also further evolves into different orthologues and transmits among different species. Data from this work suggest that Flavobacteriaceae could be the potential ancestral source of the tigecycline resistance gene tet(X).

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Epidemiological and phylogenetic analysis reveals Flavobacteriaceae as potential ancestral source of tigecycline resistance gene tet(X)

Abstract

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