Epidemiological and Genetic Characteristics of Clinical Carbapenem-Resistant Pseudomonas aeruginosa Strains in Guangdong Province, China

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Yonggang Zhao
  • Dingqiang Chen
  • Kaichao Chen
  • Jiubiao Guo
  • Edward Wai Chi Chan
  • Lu Xie
  • Jingbo Wang
  • Enqi Chen
  • Weijun Chen
  • Lars Jelsbak

Detail(s)

Original languageEnglish
Article numbere04261-22
Journal / PublicationMicrobiology Spectrum
Volume11
Issue number3
Online published20 Apr 2023
Publication statusPublished - May 2023

Link(s)

Abstract

Carbapenem-resistant Pseudomonas aeruginosa (CRPA) is a bacterial pathogen that may cause serious drug-resistant infections that are potentially fatal. To investigate the genetic characteristics of these organisms, we tested 416 P. aeruginosa strains recovered from 12 types of clinical samples collected in 29 different hospital wards in 10 hospitals in Guangdong Province, China, from 2017 to 2020. These strains were found to belong to 149 known sequence types (STs) and 72 novel STs, indicating that transmission of these strains involved multiple routes. A high rate of resistance to imipenem (89.4%) and meropenem (79.4%) and a high prevalence of pathogenic serotypes (76.4%) were observed among these strains. Six STs of global high-risk clones (HiRiCs) and a novel HiRiC strains, ST1971, which exhibited extensive drug resistance, were identified. Importantly, ST1971 HiRiC, which was unique in China, also exhibited high virulence, which alarmed the further surveillance on this highly virulent and highly resistant clone. Inactivation of the oprD gene and overexpression of efflux systems were found to be mainly responsible for carbapenem resistance in these strains; carriage of metallo-β-lactamase (MBL)-encoding genes was less common. Interestingly, frameshift mutations (49.0%) and introduction of a stop codon (22.4%) into the oprD genes were the major mechanisms of imipenem resistance. On the other hand, expression of the MexAB-OprM efflux pump and MBL-encoding genes were mechanisms of resistance in >70% of meropenem-resistant strains. The findings presented here provide insights into the development of effective strategies for control of worldwide dissemination of CRPA.

© 2023 Zhao et al.

Research Area(s)

  • Pseudomonas aeruginosa, carbapenem resistance, oprD, efflux-pump-encoding genes, molecular epidemiology, SURVEILLANCE, MECHANISMS, CYTOTOXICITY, ALGORITHM, IMIPENEM, EXOU, TREE

Citation Format(s)

Epidemiological and Genetic Characteristics of Clinical Carbapenem-Resistant Pseudomonas aeruginosa Strains in Guangdong Province, China. / Zhao, Yonggang; Chen, Dingqiang; Chen, Kaichao et al.
In: Microbiology Spectrum, Vol. 11, No. 3, e04261-22, 05.2023.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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