Enzymolysis kinetics of corn straw by impeded Michaelis model and Box-Behnken design

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Detail(s)

Original languageEnglish
Article number117658
Journal / PublicationEnvironmental Research
Volume242
Online published17 Nov 2023
Publication statusPublished - 1 Feb 2024

Abstract

Enzymatic hydrolysis is an essential step in the lignocellulosic biorefining process. In this paper, Box-Behnken was used to optimize the enzymatic hydrolysis process of corn stalk, and the promotion effect of three typical surfactants on the enzymatic hydrolysis process was investigated. The experimental results showed that the total reducing sugar yield reached 67.6% under the best-predicted conditions. When the concentration of Tween 80 is 0.1%, it could be increased to 80.2%. In addition, the Impeded Michaels Model (IMM) is introduced in this study to describe the enzymatic hydrolysis process of corn stalks. Finally, the initial contact coefficient between the enzyme and cellulose (Kobs,0) and the gradual loss coefficient of enzyme activity (ki) caused by reaction obstruction were obtained by fitting data, which successfully verified the rationality of the model. © 2023 Published by Elsevier Inc.

Research Area(s)

  • Box-Behnken, Enzymolysis, Lignocellulose, Michaelis model, Surfactant

Citation Format(s)

Enzymolysis kinetics of corn straw by impeded Michaelis model and Box-Behnken design. / Liu, Peng; Zhao, Ying; Guo, Hongliang et al.
In: Environmental Research, Vol. 242, 117658, 01.02.2024.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review