Enzyme digestion-based microfluidic system for DNA methylation assay

Chih-Hung Wang; Keng-Fu Hsu; Cheng-Yang Chou; Gwo-Bin Lee

doi:10.1109/MEMSYS.2012.6170351

Enzyme digestion-based microfluidic system for DNA methylation assay

Chih-Hung Wang
, Keng-Fu Hsu
, Cheng-Yang Chou
, Gwo-Bin Lee

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

2 Citations (Scopus)

Abstract

Early and accurate diagnosis of cancer plays a very important role in cancer treatment. The DNA methylation of tumor suppressor genes has been used as a diagnostic biomarker of early carcinogenesis. The 5-methylcytosine of CpG islands in the promoter region has been demonstrated as an evidence of DNA methylation. In this study, the entire process for performing DNA methylation assay including capturing of target DNA, HpaII and MspI endonuclease digestion and nucleic acid amplification have been realized in an integrated microfluidic system. The limit of detection in the microfluidic system was experimentally found to be 10 ² cells/reaction. The entire process from sample loading to results observed only takes 3 hrs. Rapid diagnosis of ovarian cancer cells in the integrated microfluidic system has been demonstrated by using cell lines and clinical samples. The developed micro system may be promising for early diagnosis of cancers. © 2012 IEEE.

Original language	English
Title of host publication	2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Pages	985-988
DOIs	https://doi.org/10.1109/MEMSYS.2012.6170351
Publication status	Published - 2012
Externally published	Yes
Event	25th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2012) - Paris, France Duration: 29 Jan 2012 → 2 Feb 2012

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Conference

Conference	25th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2012)
Place	France
City	Paris
Period	29/01/12 → 2/02/12

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1109/MEMSYS.2012.6170351

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Cite this

Wang, C.-H., Hsu, K.-F., Chou, C.-Y., & Lee, G.-B. (2012). Enzyme digestion-based microfluidic system for DNA methylation assay. In 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 (pp. 985-988). Article 6170351 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2012.6170351

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title = "Enzyme digestion-based microfluidic system for DNA methylation assay",

abstract = "Early and accurate diagnosis of cancer plays a very important role in cancer treatment. The DNA methylation of tumor suppressor genes has been used as a diagnostic biomarker of early carcinogenesis. The 5-methylcytosine of CpG islands in the promoter region has been demonstrated as an evidence of DNA methylation. In this study, the entire process for performing DNA methylation assay including capturing of target DNA, HpaII and MspI endonuclease digestion and nucleic acid amplification have been realized in an integrated microfluidic system. The limit of detection in the microfluidic system was experimentally found to be 10 2 cells/reaction. The entire process from sample loading to results observed only takes 3 hrs. Rapid diagnosis of ovarian cancer cells in the integrated microfluidic system has been demonstrated by using cell lines and clinical samples. The developed micro system may be promising for early diagnosis of cancers. {\textcopyright} 2012 IEEE.",

author = "Chih-Hung Wang and Keng-Fu Hsu and Cheng-Yang Chou and Gwo-Bin Lee",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].; 25th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2012) ; Conference date: 29-01-2012 Through 02-02-2012",

year = "2012",

doi = "10.1109/MEMSYS.2012.6170351",

language = "English",

isbn = "9781467303248",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

pages = "985--988",

booktitle = "2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012",

}

Wang, C-H, Hsu, K-F, Chou, C-Y & Lee, G-B 2012, Enzyme digestion-based microfluidic system for DNA methylation assay. in 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012., 6170351, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 985-988, 25th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2012), Paris, France, 29/01/12. https://doi.org/10.1109/MEMSYS.2012.6170351

Enzyme digestion-based microfluidic system for DNA methylation assay. / Wang, Chih-Hung; Hsu, Keng-Fu; Chou, Cheng-Yang et al.
2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012. 2012. p. 985-988 6170351 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Enzyme digestion-based microfluidic system for DNA methylation assay

AU - Wang, Chih-Hung

AU - Hsu, Keng-Fu

AU - Chou, Cheng-Yang

AU - Lee, Gwo-Bin

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2012

Y1 - 2012

N2 - Early and accurate diagnosis of cancer plays a very important role in cancer treatment. The DNA methylation of tumor suppressor genes has been used as a diagnostic biomarker of early carcinogenesis. The 5-methylcytosine of CpG islands in the promoter region has been demonstrated as an evidence of DNA methylation. In this study, the entire process for performing DNA methylation assay including capturing of target DNA, HpaII and MspI endonuclease digestion and nucleic acid amplification have been realized in an integrated microfluidic system. The limit of detection in the microfluidic system was experimentally found to be 10 2 cells/reaction. The entire process from sample loading to results observed only takes 3 hrs. Rapid diagnosis of ovarian cancer cells in the integrated microfluidic system has been demonstrated by using cell lines and clinical samples. The developed micro system may be promising for early diagnosis of cancers. © 2012 IEEE.

AB - Early and accurate diagnosis of cancer plays a very important role in cancer treatment. The DNA methylation of tumor suppressor genes has been used as a diagnostic biomarker of early carcinogenesis. The 5-methylcytosine of CpG islands in the promoter region has been demonstrated as an evidence of DNA methylation. In this study, the entire process for performing DNA methylation assay including capturing of target DNA, HpaII and MspI endonuclease digestion and nucleic acid amplification have been realized in an integrated microfluidic system. The limit of detection in the microfluidic system was experimentally found to be 10 2 cells/reaction. The entire process from sample loading to results observed only takes 3 hrs. Rapid diagnosis of ovarian cancer cells in the integrated microfluidic system has been demonstrated by using cell lines and clinical samples. The developed micro system may be promising for early diagnosis of cancers. © 2012 IEEE.

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UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84860480015&origin=recordpage

U2 - 10.1109/MEMSYS.2012.6170351

DO - 10.1109/MEMSYS.2012.6170351

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781467303248

T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

SP - 985

EP - 988

BT - 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012

T2 - 25th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2012)

Y2 - 29 January 2012 through 2 February 2012

ER -

Enzyme digestion-based microfluidic system for DNA methylation assay

Abstract

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Bibliographical note

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