Enzyme activities in activated sludge flocs

Guang-Hui Yu; Pin-Jing He; Li-Ming Shao; Duu-Jong Lee

doi:10.1007/s00253-007-1204-5

Enzyme activities in activated sludge flocs

Guang-Hui Yu, Pin-Jing He, Li-Ming Shao, Duu-Jong Lee

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

112 Citations (Scopus)

Abstract

This study quantified the activities of enzymes in extracellular polymeric substances (EPS) and in pellets. Seven commonly adopted extraction schemes were utilized to extract from aerobic flocs the contained EPS, which were further categorized into loosely bound (LB) and tightly bound (TB) fractions. Ultrasonication effectively extracted the EPS from sludge flocs. Enzyme assay tests showed that the protease activity was localized mainly on the pellets, α-amylase and α-glucosidase activities were largely bound with LB-EPS, and few protease, α-amylase, or α-glucosidase activities were associated with the TB-EPS fraction. There exists no correlation between the biochemical compositions of EPS and the distribution of enzyme activities in the sludge matrix. The 44-65% of α-amylase and 59-100% of α-glucosidase activities noted with the LB-EPS indicate heterogeneous hydrolysis patterns in the sludge flocs with proteins and carbohydrates. © 2007 Springer-Verlag.

Original language	English
Pages (from-to)	605-612
Journal	Applied Microbiology and Biotechnology
Volume	77
Issue number	3
DOIs	https://doi.org/10.1007/s00253-007-1204-5
Publication status	Published - Nov 2007
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 6 Clean Water and Sanitation

Research Keywords

Enzymatic distribution
Enzymes
Extracellular polymeric substances
Extraction process
Infrared spectra
Sludge

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10.1007/s00253-007-1204-5

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abstract = "This study quantified the activities of enzymes in extracellular polymeric substances (EPS) and in pellets. Seven commonly adopted extraction schemes were utilized to extract from aerobic flocs the contained EPS, which were further categorized into loosely bound (LB) and tightly bound (TB) fractions. Ultrasonication effectively extracted the EPS from sludge flocs. Enzyme assay tests showed that the protease activity was localized mainly on the pellets, α-amylase and α-glucosidase activities were largely bound with LB-EPS, and few protease, α-amylase, or α-glucosidase activities were associated with the TB-EPS fraction. There exists no correlation between the biochemical compositions of EPS and the distribution of enzyme activities in the sludge matrix. The 44-65% of α-amylase and 59-100% of α-glucosidase activities noted with the LB-EPS indicate heterogeneous hydrolysis patterns in the sludge flocs with proteins and carbohydrates. {\textcopyright} 2007 Springer-Verlag.",

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note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected]. ",

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AU - Yu, Guang-Hui

AU - He, Pin-Jing

AU - Shao, Li-Ming

AU - Lee, Duu-Jong

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2007/11

Y1 - 2007/11

N2 - This study quantified the activities of enzymes in extracellular polymeric substances (EPS) and in pellets. Seven commonly adopted extraction schemes were utilized to extract from aerobic flocs the contained EPS, which were further categorized into loosely bound (LB) and tightly bound (TB) fractions. Ultrasonication effectively extracted the EPS from sludge flocs. Enzyme assay tests showed that the protease activity was localized mainly on the pellets, α-amylase and α-glucosidase activities were largely bound with LB-EPS, and few protease, α-amylase, or α-glucosidase activities were associated with the TB-EPS fraction. There exists no correlation between the biochemical compositions of EPS and the distribution of enzyme activities in the sludge matrix. The 44-65% of α-amylase and 59-100% of α-glucosidase activities noted with the LB-EPS indicate heterogeneous hydrolysis patterns in the sludge flocs with proteins and carbohydrates. © 2007 Springer-Verlag.

AB - This study quantified the activities of enzymes in extracellular polymeric substances (EPS) and in pellets. Seven commonly adopted extraction schemes were utilized to extract from aerobic flocs the contained EPS, which were further categorized into loosely bound (LB) and tightly bound (TB) fractions. Ultrasonication effectively extracted the EPS from sludge flocs. Enzyme assay tests showed that the protease activity was localized mainly on the pellets, α-amylase and α-glucosidase activities were largely bound with LB-EPS, and few protease, α-amylase, or α-glucosidase activities were associated with the TB-EPS fraction. There exists no correlation between the biochemical compositions of EPS and the distribution of enzyme activities in the sludge matrix. The 44-65% of α-amylase and 59-100% of α-glucosidase activities noted with the LB-EPS indicate heterogeneous hydrolysis patterns in the sludge flocs with proteins and carbohydrates. © 2007 Springer-Verlag.

KW - Enzymatic distribution

KW - Enzymes

KW - Extracellular polymeric substances

KW - Extraction process

KW - Infrared spectra

KW - Sludge

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DO - 10.1007/s00253-007-1204-5

M3 - RGC 21 - Publication in refereed journal

C2 - 17932668

SN - 0175-7598

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EP - 612

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

IS - 3

ER -

Enzyme activities in activated sludge flocs

Abstract

Bibliographical note

UN SDGs

Research Keywords

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