ENHANCING/MULTIPLEXING PROTEASE ASSAY WITH DROPLET BASED MICROFLUIDICS USING BIOMOLECULE CONCENTRATOR

Chia Hung Chen; Aniruddh Sarkar; Yong-Ak Song; Miles A. Miller; Sung Jae Kim; Linda G. Griffith; Douglas A. Lauffenburger; Jongyoon Han

ENHANCING/MULTIPLEXING PROTEASE ASSAY WITH DROPLET BASED MICROFLUIDICS USING BIOMOLECULE CONCENTRATOR

Chia Hung Chen, Aniruddh Sarkar, Yong-Ak Song, Miles A. Miller, Sung Jae Kim, Linda G. Griffith, Douglas A. Lauffenburger, Jongyoon Han^*

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

We introduce an integrated microfluidic device consisting of a biomolecule concentrator, picoinjector and a micro droplet generator. We used this platform to detect ultra-low levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (∼10-fold) reduced the time required to complete the assay and the sample volume used. Biomolecule concentrator enhances the limited sensitivity of low-abundance enzyme assays by concentrating sample solutions, while droplet generator confines concentrated reactants into a pico-liter volume for sustained high sensitivity enzyme reaction as well as enabling multiplexed assays with a limited sample volume. We demonstrated to screen five different protease sensors simultaneously to indicate specific protease MMP-9 with high sensitivity.

Original language	English
Title of host publication	The15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, µTAS 2011
Editors	James P. Landers, David Juncker, Joan Bienvenue, Amy Herr, Nicole Pamme
Pages	2080-2082
ISBN (Electronic)	9780979806445
Publication status	Published - Oct 2011
Externally published	Yes
Event	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, µTAS 2011 - Washington State Convention Center, Seattle, United States Duration: 2 Oct 2011 → 6 Oct 2011 https://microtasconferences.org/microtas2011/ https://archive.cbmsociety.org/utas/uTAS2011.zip

Publication series

Name	International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS
ISSN (Electronic)	1556-5904

Conference

Conference	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, µTAS 2011
Country/Territory	United States
City	Seattle
Period	2/10/11 → 6/10/11
Internet address	https://microtasconferences.org/microtas2011/ https://archive.cbmsociety.org/utas/uTAS2011.zip

Research Keywords

Biomolecule concentrator
Emulsions
Multiplexed activity bioassay
Protease

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Chen, C. H., Sarkar, A., Song, Y.-A., Miller, M. A., Kim, S. J., Griffith, L. G., Lauffenburger, D. A., & Han, J. (2011). ENHANCING/MULTIPLEXING PROTEASE ASSAY WITH DROPLET BASED MICROFLUIDICS USING BIOMOLECULE CONCENTRATOR. In J. P. Landers, D. Juncker, J. Bienvenue, A. Herr, & N. Pamme (Eds.), The15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, µTAS 2011 (pp. 2080-2082). (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS).

Chen, Chia Hung ; Sarkar, Aniruddh ; Song, Yong-Ak et al. / ENHANCING/MULTIPLEXING PROTEASE ASSAY WITH DROPLET BASED MICROFLUIDICS USING BIOMOLECULE CONCENTRATOR. The15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, µTAS 2011. editor / James P. Landers ; David Juncker ; Joan Bienvenue ; Amy Herr ; Nicole Pamme. 2011. pp. 2080-2082 (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS).

@inproceedings{e4ebf3866e524391b3dce6472287de74,

title = "ENHANCING/MULTIPLEXING PROTEASE ASSAY WITH DROPLET BASED MICROFLUIDICS USING BIOMOLECULE CONCENTRATOR",

abstract = "We introduce an integrated microfluidic device consisting of a biomolecule concentrator, picoinjector and a micro droplet generator. We used this platform to detect ultra-low levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (∼10-fold) reduced the time required to complete the assay and the sample volume used. Biomolecule concentrator enhances the limited sensitivity of low-abundance enzyme assays by concentrating sample solutions, while droplet generator confines concentrated reactants into a pico-liter volume for sustained high sensitivity enzyme reaction as well as enabling multiplexed assays with a limited sample volume. We demonstrated to screen five different protease sensors simultaneously to indicate specific protease MMP-9 with high sensitivity.",

keywords = "Biomolecule concentrator, Emulsions, Multiplexed activity bioassay, Protease",

author = "Chen, {Chia Hung} and Aniruddh Sarkar and Yong-Ak Song and Miller, {Miles A.} and Kim, {Sung Jae} and Griffith, {Linda G.} and Lauffenburger, {Douglas A.} and Jongyoon Han",

year = "2011",

month = oct,

language = "English",

isbn = "9781618395955",

series = "International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS",

pages = "2080--2082",

editor = "Landers, {James P.} and David Juncker and Joan Bienvenue and Amy Herr and Nicole Pamme",

booktitle = "The15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, µTAS 2011",

note = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, µTAS 2011 ; Conference date: 02-10-2011 Through 06-10-2011",

url = "https://microtasconferences.org/microtas2011/, https://archive.cbmsociety.org/utas/uTAS2011.zip",

}

Chen, CH, Sarkar, A, Song, Y-A, Miller, MA, Kim, SJ, Griffith, LG, Lauffenburger, DA & Han, J 2011, ENHANCING/MULTIPLEXING PROTEASE ASSAY WITH DROPLET BASED MICROFLUIDICS USING BIOMOLECULE CONCENTRATOR. in JP Landers, D Juncker, J Bienvenue, A Herr & N Pamme (eds), The15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, µTAS 2011. International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS, pp. 2080-2082, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, µTAS 2011, Seattle, Washington, United States, 2/10/11.

ENHANCING/MULTIPLEXING PROTEASE ASSAY WITH DROPLET BASED MICROFLUIDICS USING BIOMOLECULE CONCENTRATOR. / Chen, Chia Hung; Sarkar, Aniruddh; Song, Yong-Ak et al.
The15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, µTAS 2011. ed. / James P. Landers; David Juncker; Joan Bienvenue; Amy Herr; Nicole Pamme. 2011. p. 2080-2082 (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - ENHANCING/MULTIPLEXING PROTEASE ASSAY WITH DROPLET BASED MICROFLUIDICS USING BIOMOLECULE CONCENTRATOR

AU - Chen, Chia Hung

AU - Sarkar, Aniruddh

AU - Song, Yong-Ak

AU - Miller, Miles A.

AU - Kim, Sung Jae

AU - Griffith, Linda G.

AU - Lauffenburger, Douglas A.

AU - Han, Jongyoon

PY - 2011/10

Y1 - 2011/10

N2 - We introduce an integrated microfluidic device consisting of a biomolecule concentrator, picoinjector and a micro droplet generator. We used this platform to detect ultra-low levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (∼10-fold) reduced the time required to complete the assay and the sample volume used. Biomolecule concentrator enhances the limited sensitivity of low-abundance enzyme assays by concentrating sample solutions, while droplet generator confines concentrated reactants into a pico-liter volume for sustained high sensitivity enzyme reaction as well as enabling multiplexed assays with a limited sample volume. We demonstrated to screen five different protease sensors simultaneously to indicate specific protease MMP-9 with high sensitivity.

AB - We introduce an integrated microfluidic device consisting of a biomolecule concentrator, picoinjector and a micro droplet generator. We used this platform to detect ultra-low levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (∼10-fold) reduced the time required to complete the assay and the sample volume used. Biomolecule concentrator enhances the limited sensitivity of low-abundance enzyme assays by concentrating sample solutions, while droplet generator confines concentrated reactants into a pico-liter volume for sustained high sensitivity enzyme reaction as well as enabling multiplexed assays with a limited sample volume. We demonstrated to screen five different protease sensors simultaneously to indicate specific protease MMP-9 with high sensitivity.

KW - Biomolecule concentrator

KW - Emulsions

KW - Multiplexed activity bioassay

KW - Protease

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UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84883781164&origin=recordpage

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781618395955

T3 - International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS

SP - 2080

EP - 2082

BT - The15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, µTAS 2011

A2 - Landers, James P.

A2 - Juncker, David

A2 - Bienvenue, Joan

A2 - Herr, Amy

A2 - Pamme, Nicole

T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, µTAS 2011

Y2 - 2 October 2011 through 6 October 2011

ER -

Chen CH, Sarkar A, Song YA, Miller MA, Kim SJ, Griffith LG et al. ENHANCING/MULTIPLEXING PROTEASE ASSAY WITH DROPLET BASED MICROFLUIDICS USING BIOMOLECULE CONCENTRATOR. In Landers JP, Juncker D, Bienvenue J, Herr A, Pamme N, editors, The15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, µTAS 2011. 2011. p. 2080-2082. (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS).

ENHANCING/MULTIPLEXING PROTEASE ASSAY WITH DROPLET BASED MICROFLUIDICS USING BIOMOLECULE CONCENTRATOR

Abstract

Publication series

Conference

Research Keywords

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