Engineering bacterial two-component system PmrA/PmrB to sense lanthanide ions

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

19 Scopus Citations
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Author(s)

  • Haihua Liang
  • Mike Bosscher
  • Quanjiang Ji
  • Mark P. Jensen
  • Chuan He

Detail(s)

Original languageEnglish
Pages (from-to)2037-2039
Journal / PublicationJournal of the American Chemical Society
Volume135
Issue number6
Online published27 Jan 2013
Publication statusPublished - 13 Feb 2013
Externally publishedYes

Abstract

The Salmonella PmrA/PmrB two-component system uses an iron(III)-binding motif on the cell surface to sense the environmental or host ferric level and regulate PmrA-controlled gene expression. We replaced the iron(III)-binding motif with a lanthanide-binding peptide sequence that is known to selectively recognize trivalent lanthanide ions. The newly engineered two-component system (PmrA/PmrB) can effectively sense lanthanide ion and regulate gene expression in E. coli. This work not only provides the first known lanthanide-based sensing and response in live cells but also demonstrates that the PmrA/PmrB system is a suitable template for future synthetic biology efforts to construct bacteria that can sense and respond to other metal ions in remediation or sequestration. © 2013 American Chemical Society.

Citation Format(s)

Engineering bacterial two-component system PmrA/PmrB to sense lanthanide ions. / Liang, Haihua; Deng, Xin; Bosscher, Mike et al.

In: Journal of the American Chemical Society, Vol. 135, No. 6, 13.02.2013, p. 2037-2039.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review