Engineered living hydrogels for robust biocatalysis in pure organic solvents

Liang Gao; Lilin Feng; Daniel F. Sauer; Malte Wittwer; Yong Hu; Johannes Schiffels; Xin Li

doi:10.1016/j.xcrp.2022.101054

Engineered living hydrogels for robust biocatalysis in pure organic solvents

Liang Gao (Co-first Author)
, Lilin Feng (Co-first Author)
, Daniel F. Sauer
, Malte Wittwer
, Yong Hu^*
, Johannes Schiffels^*
, Xin Li^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

20 Citations (Scopus)

Abstract

Engineered living hydrogels that can protect cells from harsh environments have achieved preliminary successes in biomedicine and environmental remediation. However, their biocatalytic applications in pure organic solvents have not been explored. Here, living hydrogels were engineered by integrating genetically modified Escherichia coli cells into alginate hydrogels for robust biocatalysis in pure organic solvents. The biocompatible hydrogels could not only support cell growth and diminish cell escape but could also act as protective matrices to improve organic solvent tolerance, thereby prolonging catalytic activity of whole-cell biocatalysts. Moreover, the influence of hydrogel microenvironments on biocatalytic efficiency was thoroughly investigated. Importantly, the versatility of engineered living hydrogels paves the way to achieve robust biocatalytic efficiency in a variety of pure organic co-solvents. Overall, we are able to engineer living hydrogels for regio-selective synthesis in pure organic solvents, which may be particularly useful for the innovation of living hydrogels in biocatalysis.

© 2022 The Author(s)

Original language	English
Article number	101054
Number of pages	12
Journal	Cell Reports Physical Science
Volume	3
Issue number	10
Online published	12 Sept 2022
DOIs	https://doi.org/10.1016/j.xcrp.2022.101054
Publication status	Published - 19 Oct 2022
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2022 The Author(s)

Funding

This research was funded by the National Natural Science Foundation of China (52103181), Sino-German Center for Research Promotion (GZ1505), and Fundamental Research Funds for the Central Universities (22120210364). L.G. and L.F. are supported by the China Scholarship Council (CSC nos. 201708330280 and 201708330279).

Research Keywords

alginate
Escherichia coli
hydroxylation
living hydrogels
organic solvent tolerance

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title = "Engineered living hydrogels for robust biocatalysis in pure organic solvents",

abstract = "Engineered living hydrogels that can protect cells from harsh environments have achieved preliminary successes in biomedicine and environmental remediation. However, their biocatalytic applications in pure organic solvents have not been explored. Here, living hydrogels were engineered by integrating genetically modified Escherichia coli cells into alginate hydrogels for robust biocatalysis in pure organic solvents. The biocompatible hydrogels could not only support cell growth and diminish cell escape but could also act as protective matrices to improve organic solvent tolerance, thereby prolonging catalytic activity of whole-cell biocatalysts. Moreover, the influence of hydrogel microenvironments on biocatalytic efficiency was thoroughly investigated. Importantly, the versatility of engineered living hydrogels paves the way to achieve robust biocatalytic efficiency in a variety of pure organic co-solvents. Overall, we are able to engineer living hydrogels for regio-selective synthesis in pure organic solvents, which may be particularly useful for the innovation of living hydrogels in biocatalysis. {\textcopyright} 2022 The Author(s)",

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author = "Liang Gao and Lilin Feng and Sauer, \{Daniel F.\} and Malte Wittwer and Yong Hu and Johannes Schiffels and Xin Li",

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AU - Gao, Liang

AU - Feng, Lilin

AU - Sauer, Daniel F.

AU - Wittwer, Malte

AU - Hu, Yong

AU - Schiffels, Johannes

AU - Li, Xin

PY - 2022/10/19

Y1 - 2022/10/19

N2 - Engineered living hydrogels that can protect cells from harsh environments have achieved preliminary successes in biomedicine and environmental remediation. However, their biocatalytic applications in pure organic solvents have not been explored. Here, living hydrogels were engineered by integrating genetically modified Escherichia coli cells into alginate hydrogels for robust biocatalysis in pure organic solvents. The biocompatible hydrogels could not only support cell growth and diminish cell escape but could also act as protective matrices to improve organic solvent tolerance, thereby prolonging catalytic activity of whole-cell biocatalysts. Moreover, the influence of hydrogel microenvironments on biocatalytic efficiency was thoroughly investigated. Importantly, the versatility of engineered living hydrogels paves the way to achieve robust biocatalytic efficiency in a variety of pure organic co-solvents. Overall, we are able to engineer living hydrogels for regio-selective synthesis in pure organic solvents, which may be particularly useful for the innovation of living hydrogels in biocatalysis. © 2022 The Author(s)

AB - Engineered living hydrogels that can protect cells from harsh environments have achieved preliminary successes in biomedicine and environmental remediation. However, their biocatalytic applications in pure organic solvents have not been explored. Here, living hydrogels were engineered by integrating genetically modified Escherichia coli cells into alginate hydrogels for robust biocatalysis in pure organic solvents. The biocompatible hydrogels could not only support cell growth and diminish cell escape but could also act as protective matrices to improve organic solvent tolerance, thereby prolonging catalytic activity of whole-cell biocatalysts. Moreover, the influence of hydrogel microenvironments on biocatalytic efficiency was thoroughly investigated. Importantly, the versatility of engineered living hydrogels paves the way to achieve robust biocatalytic efficiency in a variety of pure organic co-solvents. Overall, we are able to engineer living hydrogels for regio-selective synthesis in pure organic solvents, which may be particularly useful for the innovation of living hydrogels in biocatalysis. © 2022 The Author(s)

KW - alginate

KW - Escherichia coli

KW - hydroxylation

KW - living hydrogels

KW - organic solvent tolerance

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DO - 10.1016/j.xcrp.2022.101054

M3 - RGC 21 - Publication in refereed journal

AN - SCOPUS:85140732891

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JO - Cell Reports Physical Science

JF - Cell Reports Physical Science

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ER -

Engineered living hydrogels for robust biocatalysis in pure organic solvents

Abstract

Bibliographical note

Funding

Research Keywords

Publisher's Copyright Statement

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