ENABLING RELIABLE DETECTION OF LOW ABUNDANCE MALARIA PARASITES FROM BLOOD USING INERTIAL MICROFLUIDICS

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review

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Author(s)

  • M. Ebrahimi Warkiani
  • A.K.P. Tay
  • X. Xiaofeng
  • C.T. Lim
  • J. Han

Detail(s)

Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014)
PublisherChemical and Biological Microsystems Society
Pages1157-1159
Volume1
ISBN (Electronic)9780979806476
ISBN (Print)9781634396974
Publication statusPublished - Oct 2014
Externally publishedYes

Conference

Title18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014)
PlaceUnited States
CitySan Antonio
Period26 - 30 October 2014

Abstract

In this paper, a highly efficient inertial microfluidic device was described to enable the enrichment and purification of malaria parasites from blood, which leads to more reliable and specific PCR-based malaria detection. The device makes use of equilibrium between shear-modulated inertial lift and wall-induced lift forces to remove WBCs, thereby facilitating malaria parasite enrichment. The cascaded system is able to process 1 mL of lysed blood in 15 min, with the WBC depletion efficiency of 99.99% which is higher than any commercially available kit (i.e., CD-45 coated beads) for this purpose. Parasite densities ranging from 103 to 104 P. falciparum parasites per mL (∼ 2 per μL) have been quantified in whole blood using quantitative PCR. Obtained results revealed that the sample preparation using the inertial microfluidic device can significantly enhance the PCR results and make it more reliable by removing most of the unwanted genomic materials and PCR inhibitors.

Research Area(s)

  • Malaria, Microfluidics, PCR, Point-of-care

Citation Format(s)

ENABLING RELIABLE DETECTION OF LOW ABUNDANCE MALARIA PARASITES FROM BLOOD USING INERTIAL MICROFLUIDICS. / Warkiani, M. Ebrahimi; Tay, A.K.P.; Khoo, B.L. et al.

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014). Vol. 1 Chemical and Biological Microsystems Society, 2014. p. 1157-1159.

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review