Elucidation and Heterologous Reconstitution of Chrodrimanin B Biosynthesis

Tongxuan Bai; Zhiyang Quan; Rui Zhai; Takayoshi Awakawa; Yudai Matsuda; Ikuro Abe

doi:10.1021/acs.orglett.8b03268

Elucidation and Heterologous Reconstitution of Chrodrimanin B Biosynthesis

Tongxuan Bai, Zhiyang Quan, Rui Zhai, Takayoshi Awakawa, Yudai Matsuda^*, Ikuro Abe^*

^*Corresponding author for this work

Department of Chemistry

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

48 Citations (Scopus)

Abstract

The biosynthetic gene cluster of the fungal meroterpenoid chrodrimanin B (4) was discovered in Penicillium verruculosum TPU1311, and the complete biosynthetic pathway of 4 has been elucidated by heterologous reconstitution of its biosynthesis in Aspergillus oryzae, as well as by in vitro characterizations of selected enzymes. The present study has identified the polyketide synthase that produces 6-hydroxymellein (3) and also provided a biosynthetic platform of chrodrimanins for further bioengineering.

Original language	English
Pages (from-to)	7504−7508
Journal	Organic Letters
Volume	20
Issue number	23
Online published	12 Nov 2018
DOIs	https://doi.org/10.1021/acs.orglett.8b03268
Publication status	Published - 7 Dec 2018

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abstract = "The biosynthetic gene cluster of the fungal meroterpenoid chrodrimanin B (4) was discovered in Penicillium verruculosum TPU1311, and the complete biosynthetic pathway of 4 has been elucidated by heterologous reconstitution of its biosynthesis in Aspergillus oryzae, as well as by in vitro characterizations of selected enzymes. The present study has identified the polyketide synthase that produces 6-hydroxymellein (3) and also provided a biosynthetic platform of chrodrimanins for further bioengineering.",

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T1 - Elucidation and Heterologous Reconstitution of Chrodrimanin B Biosynthesis

AU - Bai, Tongxuan

AU - Quan, Zhiyang

AU - Zhai, Rui

AU - Awakawa, Takayoshi

AU - Matsuda, Yudai

AU - Abe, Ikuro

PY - 2018/12/7

Y1 - 2018/12/7

N2 - The biosynthetic gene cluster of the fungal meroterpenoid chrodrimanin B (4) was discovered in Penicillium verruculosum TPU1311, and the complete biosynthetic pathway of 4 has been elucidated by heterologous reconstitution of its biosynthesis in Aspergillus oryzae, as well as by in vitro characterizations of selected enzymes. The present study has identified the polyketide synthase that produces 6-hydroxymellein (3) and also provided a biosynthetic platform of chrodrimanins for further bioengineering.

AB - The biosynthetic gene cluster of the fungal meroterpenoid chrodrimanin B (4) was discovered in Penicillium verruculosum TPU1311, and the complete biosynthetic pathway of 4 has been elucidated by heterologous reconstitution of its biosynthesis in Aspergillus oryzae, as well as by in vitro characterizations of selected enzymes. The present study has identified the polyketide synthase that produces 6-hydroxymellein (3) and also provided a biosynthetic platform of chrodrimanins for further bioengineering.

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U2 - 10.1021/acs.orglett.8b03268

DO - 10.1021/acs.orglett.8b03268

M3 - RGC 21 - Publication in refereed journal

SN - 1523-7060

VL - 20

SP - 7504−7508

JO - Organic Letters

JF - Organic Letters

IS - 23

ER -

Elucidation and Heterologous Reconstitution of Chrodrimanin B Biosynthesis

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