Electrophysiological measurements of synaptic connectivity and plasticity in the longitudinal dentate gyrus network from mouse hippocampal slices

Yoon Ji Kwon; Sojeong Pak; Sunggu Yang; Sungchil Yang

doi:10.1016/j.xpro.2022.102030

Author(s)

Related Research Unit(s)

Department of Neuroscience

Detail(s)

Original language	English
Article number	102030
Journal / Publication	STAR Protocols
Volume	4
Issue number	1
Online published	11 Jan 2023
Publication status	Published - 17 Mar 2023

Link(s)

DOI	DOI https://doi.org/10.1016/j.xpro.2022.102030 Final Published version
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Attachment(s)	Documents 128279784.pdf Final Published version, 3.71 MB, PDF Publisher's Copyright Statement This full text is made available under CC-BY 4.0. https://creativecommons.org/licenses/by/4.0/
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-85146136311&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(903adb1a-8ab0-44c5-85e5-e40fac7bf55c).html

Abstract

Longitudinal synaptic connections between dentate gyrus (DG) granule neurons in the hippocampus have been found to be correlated with increased anxiety. Here, we present a protocol to assess synaptic connectivity and plasticity in the longitudinal DG network. We detail the steps for (1) obtaining acute mouse hippocampal slices that contain longitudinal DG-DG connections, (2) measuring excitatory postsynaptic potentials using whole-cell patch clamp recording combined with two-photon microscopy and glutamate uncaging, and (3) assessing synaptic plasticity using extracellular field recording.

For complete details on the use and execution of this protocol, please refer to Pak et al. (2022).¹